The Research Of Chemical Composition And Quality Standard For Radix Dolomiaeae

Radix Dolomiaeae is the dried rhizome of Vladimiria souliei(Franch.)Ling.and Vladimiria souliei(Franch.)Ling vat.cinerea Ling.It was used as a succedaneum of Radix Aucklandiae for a long time.1963,began to be"chinese pharmacopoeia"singal collection until 2005. It is mainly produced in Sichuan.It is uses to relieving Qi and relieve pain as Chinese traditional medicine,but also Tibetan, Qiang, Mongolia, habitual medicine, mainly for digestive diseases.There were many varieties of plants which were used as Mu-xiang in history,and the name changed several times,leading to the Clinical application of chaos. From the textual research of materia medica,members of this project investigated the resources,effect,processing,carried out its chemical constituents.Then assayed Costunolide,Dehydrocostus lactone and other three compounds which obtained by ourself by HPLC. Initially established its qu-ality standards by chromatographic fingerprint.1 materia medica herbal textual research of Mu-xiang andThrough access to a large number of ancient literature,we did complete inspection and sorting on its species and names of the historical changes. Clarified the relationships between the various alternatives and the confused.2 Chemical constituents of Radix Dolomiaeae Silica gel column chromatography was used to analysis Radix Dolomiaeae.We got 11 compounds from it ,α-amyrin margarate(Ⅰ),Isobauerenyl acetate(Ⅱ),Dehydrocostus lactone(Ⅲ),Uvaol(Ⅳ),15-Acetoxycostunolide(Ⅴ),Santamarine(Ⅵ),Prinsepiol(Ⅶ),Syringaresinol(Ⅷ),VladinolD(Ⅸ),Costunolide(Ⅹ)and Cynaropicrin(Ⅺ)。except Costunolide,VladinolD and Dehydrocostus lactone are known active ingredients,others are found in Radix Dolomiaeae the first time.3 Quality Standards of Radix DolomiaeaeBy using the method of HPLC to determine the content of Dehydrocostus lactone,Costunolide,bauerenyl acetate,Syringaresinol and Cynaropicrin.The results showed that there are certain distinctions between the content of these compounds. By using the method of HPLC, cost-unolide be the index , we conducted fingerprint on Radix Dolomiaeae, established its quality standards initially.