The Clinical And Experimental Research Of Jia Wei Sheng Mai Yin On Coronary Heart Disease

Objective:To study the clinical effect of Jia Wei Sheng Mai Yin (JWSMY) on the patients of coronary, and inquire into the mechanism of experimental atherosclerosis in Rabbits that its Resistance to atherosclerosis.Methods:1.Clinical research:60 patiens of CHD with stable tired angina were chosen and divided into 2 groups randomly,one group were treated with anti-angina drugs and JWSMY as experiment group ,while the other group were treated with anti-angina drugs as control group.Three months later,we observed the effet on the patiens.2. Experimental Study: 24 New Zealand white rabbits were randomly divided into three groups, n = 8. The first group were fed a high cholesterol diet; the second group with JWSMY (1g·d^-1·Kg^-1), at the same time the rabbits were fed a high cholesterol diet; the third group to a normal diet. Three groups were fed four months later,we took some blood from the ear margin veins, and used enzymology methods to determine each animal blood lipid levels.Then we killed animals, took chest aortas, and made HE staing and MASSON staing to observe atherosclerosis and spot mass fiber.Also we used immunity organization chemistry to observe smooth muscle cells protein expression.Results:1.Clinical research:Compared with control, the treatment group angina illness integral significantly reduced (P<0.05) after treatment,and the angina curative effect improved (P<0.05). TCM symptoms integral decreased obviously (P<0.05), and TCM symptoms improved efficacy significant (P<0.05). All of them could explain that JWSMY markedly improve CHD patients, angina symptoms, and signs. Two groups compared, the electrocardiogram curative effect of treatment group (the effective rate 76.67%)obviously improved than that in control group (the effective rate43.33 % ). In the course of treatment, both groups were not found fever, hemorrhage, skin rash, adverse reactions. During the treatment, the liver, kidney and three conventional check and also have no abnormal findings.This method treatment is safe and reliable. 2.Experimental research shows that: (1) Comparison of three groups of lipids:The degree of atherosclerosis was significantly lower in JWSMY group than that in high-cholesterol group.(2) Three degree of atherosclerosis in comparison:The intima thickness and intima-media thickness ratio (I/M thickness) of JWSMY group were significantly reduced than the high-cholesterol group (P< 0.05),and plaques fiber content increased obviously (P<0.05).(3)Three groups of smooth muscle cell protein expression: Compared with the high-cholesterol group,JWSMY group decreased significantly (P<0.05), and the normal control group without expression.Conclusions:1.JWSMY treatment of coronary heart disease chronic stable angina (Qi and Yin deficiency syndrome) curative effect is distinct.2. JWSMY can significantly inhibited the protein expression of smooth muscle cells in AS. 3. JWSMY can significantly reduce the degree of atherosclerosis.