Expression Of P53 In Retina Of CYP1B1 Knock- Out Mice

Objective To investigate the significance and expression of P53 in the retina of CYP1B1 gene knock out mice .Method CYP1B1 gene knock out mice aged from twelve to sixteen weeks were taken as the experimental group(n=15) , C57BL/6J mice of the same age served as control(n=15). We compared their behavior to light and observed the corneal shape roughly. The intraocular pressure (IOP) of mice was measured by Tono-penXL applanometer. Paraffin imbedding and sagittally section technique were used for observing the morphology of retina and counting the number of retinal ganglial cells (RGCs) by Image-Pro Plus 6.0 analytical system. The expression of P53 in mice retina was detected by immunohistochemistry and reverse transcriptase - polymerase chain reaction (RT - PCR) technique.Results Both of the two groups showed the normal behavior to light, no obvious disorder of cornea was found. There were no significant differences of IOP and numbers of RGCs between two groups. No obvious morphologic change of retina was found under the light microscope in both groups mice. Compared with the control group, the expression of the P53 increased in the retinal of the CYP1B1 gene knock out mice (P < 0. 05).Conclusion P53 may be involved in the change of susceptivity to glaucoma induced by CYP1B1 gene knocked out.