Screening Of Effective Fractions And Pharmacodynamical Study On Liver Protective Activity Of Capparis Spinosa L.Fruits

Capparis spinosa L., with high ecological value, is mainly distributed in Mediterranean region and Arabia, it's also distributed in Sinkiang and Tibet of China. It has various pharmacological activities, and can be used for both food and medicine. The pharmacological value of C. spinosa has been researched extensively abroad. C. spinosa is a kind of traditional herbal medicine with long history of application, but its antihepatotoxic activity had never been reported in our country. In order to provide new ideas and methods for developing clinical value of C. spinosa and screening new medicine for liver diseases, we selected effective fractions against acute liver injury from fruits of C. spinosa from Sinkiang, and evaluated its protective effect on different liver injuries.(1)Through alalyzing contents of transaminases, SOD, MDA and changes of histomorphology in mice whose liver were injured by carbon tetrachloride, we found that the effective fractions for protecting liver focused on water extract and alcohol extract.(2)Methanol-soluble fractions from water extract of C. spinosa had a highly preventive effect on acute liver injury in mice. Results indicated that the extract could decrease contents of transaminases and MDA, increase content of SOD in liver and improve the pathological liver injuries. Scavenging free radicals and reducing lipid peroxidation were supposed to be one of possible mechanism.(3) C. spinosa could protect liver against injury induced by alcohol. The results showed that the material could lower contents of transaminases, total cholesterol and MDA in liver, raise levels of serum Alb, total protein and SOD.(4) C. spinosa indicated a better therapeutic effect on liver fibrosis in rats induced by carbon tetrachloride and alcohol. After six week's oral administration, compared with model group, drug groups showed a highly reduction of transaminases, TBil, MDA and Hyp, as well as the obviously increasing of Alb and SOD. So C. spinosa could improve state of fibrosis proliferation and pathological injury.