Effect Of Panax Nologinoside On Expression Of TGF-β1 And Smad-7 In Rats With Type 1 Diabeticnephropathy

Objective:To explore the protective function and molecular mechanism of Panax Nologinoside(PNS) on kidneys in diabetic rats by investigating the effect of PNS on expression of transforming growth factor-β1﹙TGF-β1﹚and Smad-7 in rats kidneys with type 1 diabetes. Method:50 male Wistar rats were randomized into 5 groups,which included normal group(N group),diabetes model group(DM group),low dose PNS-treated group(PNS-L group),high dose PNS-treated group(PNS-H group)and captopril-treated group(C group).Type 1 Diabetes rat model was established by single tail-vein injection of streptozotocin.After that the rat stomach had been perfused with distilled water,distilled water,PNS 100mg·kg-1·d-1,PNS 200 mg·kg-1·d-1 or captopril 50mg·kg-1·d-1 in Group N,DM,PNS-L,PNS-H or C respectively for six weeks, the fasting blood glucose(FBG),24h urinary albumin,serum creatinine and urine creatinine were examined and TGF-β1 , Smad7 level in rats kidneys were tested by immunohistochemistry.Result:﹙1﹚By comparing to normal group﹙N group﹚, FBG﹙P<0.01﹚,24h urinary albumin﹙P<0.01﹚,creatinine clearance rate﹙CCr﹚﹙P<0.01﹚ in diabetes model group were markedly increased.﹙2﹚By comparing to diabetes model group﹙DM group﹚, FBG﹙P<0.01﹚,24h urinary albumin﹙P<0.01﹚,creatinine clearance rate﹙CCr﹚﹙P<0.01﹚ in PNS -treated groups﹙PNS-L group,PNS-H group﹚ were significantly decreased.﹙3﹚By comparing to normal group﹙N group﹚, TGF-β1﹙P<0.01﹚,Smad7﹙P<0.01﹚ in diabetes model group were markedly increased.﹙4﹚By comparing to diabetes model group﹙DM group﹚, whereas TGF-β1﹙P<0.05﹚in PNS-treated groups﹙PNS-L group,PNS-H group﹚ were significantly decreased, Smad7﹙P<0.05﹚expression in PNS-treated groups﹙PNS-L group,PNS-H group﹚were markedly increased. Conclusion:Panax Nologinoside exerted protective effect on diabetic nephropathy via reduction of FBG,24h urinary albumin and creatinine clearance rate.The mechanism of Panax Nologinoside could be related to reduction of TGF-β1 and elevation of Smad7.The present research not only investigates the potentiality of Panax Nologinoside to be a curative for diabetic nephropathy, but also explain the possible mechanism of Panax Nologinoside by molecular level , providing evidence for Panax Nologinoside as an clinal curative for diabetic nephropathy.