| Objective: To investigate the expression of NF-κB p50 and IκBαin the amph-hematoma of rats with experimental intracerebral hemorrhage and the effects of Erythropoietin(EPO) on them, to study the depressant effects and mechanism of erythropoietin to inflammatory reaction. Method: 1.One hundred and twenty-six rats were randomly divided into 3 groups, i.e. sham operation group(SOG), intracerebral hemorrhage group(IHG) and EPO group(EPOG).There were forty-two rats in SOG,IHG and EPOG. There were seven subsets in this three groups, i.e. the 3rd,6th,12th,24th,48th and 72th hours and 7th days. There were six rats in each subset.2. We had injected autologous blood 50μl into the right caudate nucleus in rats to make an model of intracerebral hemorrhage.3. We studied neurologic function changes after intracerebral hemorrhage of rats,morphology changes under light microscope,the degree of brain edema,the expression of NF-κBp50 and IκBαin cortex and neuron apoptosis respectively by using neurologic function score, HE staining, dry/wet weight method , immunohistochemistry and TUNEL.4. We compared the changes of brain edema degree, morphology , NF-κBp50,IκBαexpression and neuron apoptosis in each group in seven subsets.5. We arranged the date by the EXCEL database and analysised the date by SPSS13.0 statistics software.We expressed the mean of mV and var as mean±SD.We used the variance analysis of single factor between interclass.Results:1.The neurologic deficit scores of EPOG (2.36±0.18) was lower than that of IHG(3.67±0.48),and there was the significant difference(p<0.05).2. The brain water content progressively increased within the 72th hour of intracerebral hemorrhage. The brain water content, , expressions of NF-κB p50 significantly decreased and expressions of IκBαsignificantly increased in EPOG compared with IHG in seven subsets(p<0.05). 3. Under light microscopy,There was a conspicuous blood tumor like oval-shap in caudate nucleus place after 24h of operation.There were dispersed necrotic cells in the center of the blood tumor and obvious dropsy,necrotic nerve cells,heterophil granulocyte in the region around hematoma.The dropsy beacause of inflammatory reaction in the region around hematoma and the inflammatory cells decreased in EPOG on the 24th hour.4. The expression of NF-κBp50 and IκBαwas detected to increase at the 6th hour following the operation, continuing to increase significantly on the 24th hour, getting to the peak level on the 72th hour. To compare with SOG the expression of NF-κB p50 of IHG were increased significantly ( P<0·01). The expression of NF-κB p50 in EPOG were lower than that in IHG(P<0.01). The expression of IκBαwas obviously up-regulated in the rats treated by EPO(P<0.01).5. There were slight apoptotic cells in SOG.The apoptotic cells started to increase after the 3rd hour of intracerebral hemorrhage and obviously increased after the 6th hour of intracerebral hemorrhage.There was the significant difference between IHG and SOG in seven subsets(p<0.01). The apoptotic cells significantly decreased in EPOG,and there was the significant difference compared with the apoptotic cells in IHG in the 12th,24th,48th,72th hours and 7th days(p<0.05, p<0.01).Conclusion: 1. After intracerebral hemorrhage, there were obvious inflammatory reaction and apoptosis in the edge of blood tumor,and they had evident timeliness, reached peak after the 72th hour. The inflammatory reaction and the dropsy beacause of inflammatory reaction and the apoptosis in the region around hematoma could be restrained by EPO.2.The expression of NF-κB p50 and IκBαwas detected to increase in thebrain damage after experimental intracerebral hemorrhage.EPO could reduce the expression of NF-κB p50 and enhance the expression of IκBα. |
