The Relationship Between The HBVcccDNA Quantitative Of Liver Tissues And Il-10, Ifn-γ, TGF-β1 Level Of The Serum In The Patients With Chronic Hepatitis B

Objective In this research,we detected the quantitative in liver tissue of covalently closed circular DNA (cccDNA)on chronic hepatitis B (CHB) patients and quantification of serum IL-10, IFN-γ, TGF-β1. To explore the important value of cccDNA in diagnosis of the CHB and the relationship between immune. Methods We use fluorescence quantitative polymerase chain reaction (FQ-PCR) to detect liver HBV cccDNA, liver tissue and serum HBV DNA levels. At the same time using enzyme-linked immunosorbent assay (ELISA) to detect the levels of IFN-γ, IL-10, TGF-β1 in serum. Using statistical analysis software with spss11.5 to analyse the relationship among the liver tissue HBV cccDNA and HBV DNA load , serum HBV DNA load and the serum IFN-γ, IL-10,TGF-β1 levels. Also analyzed the mutual relations among serum IFN-γ, IL-10, TGF-β1 level. Result①40 cases of 42 cases patients'liver tissues HBV cccDNA were positive, accounting for 95.24%; All patients'liver HBV DNA were positive,the detection rate was 100%. And the detection rate of HBV DNA in serum was 95.24%. Above them 4 cases whose serum HBV DNA were negative but positive in liver tissue, and 2 case of HBV cccDNA was positive. The 5 cases of normal control group, the HBV cccDNA, HBV DNA in liver and HBV DNA in serum were negative.②The load of liver HBV cccDNA and HBV DNA was positively correlated(r=0.856,P<0.01),and serum HBV DNA was positive correlation too(r=0.602,P<0.01). The load of HBV DNA in liver tissue and HBV DNA in serum was positive correlation(r=0.745,P<0.01);③The serum IFN-γin 66 patients were significantly lower than the control group and the serum IL-10 and TGF-β1 were significantly higher (P all <0.05); There were no difference between LC group and CHB group about serum IFN-γand IL-10(P> 0.05). And significant difference about serum TGF-β1 between the CHB and the LC group(P<0.05);④The 42 patients'liver HBV cccDNA loads and serum IFN-γlevel was negatively correlated (r= -0.679, P<0.01); and IL-10 was positively correlated (r= 0.723, P<0.01); but with TGF-β1 was no significant correlation (r = -0.125, P> 0.1); The load of liver HBV DNA and serum IFN-γwas negatively correlated (r = -0.523, P<0.01); and IL-10 was positively correlated (r= 0.657, P<0.01); but with TGF-β1 was no significant correlation (r= -0.158, P> 0.1). The load of serum HBV DNA and serum IFN-γwas no significant correlation (r= -0.198, P>0.05); and IL-10 was positively correlated (r= 0.458, P<0.01); with TGF-β1 was no significant correlation (r = -0.091, P>0.1). Conclusion The load of liver HBV cccDNA is more accurately reflect the hepatitis B virus infection in the liver cells and replicating the state level than serum HBV DNA. When the load of serum HBV DNA too low to detect, the virus may stil replication in liver tissue. The load of liver HBV cccDNA, HBV DNA, serum HBV DNA and serum IFN-γ, IL-10 levels are correlated, which indicated the active and persistent infection with the virus is not only depend on the virus's biological characteristics, but also to the state of host's immune function.