Effection Of Repairing Of Radial Defect By Antigenicity Of BMSCs Of Homogeneity Variant Rabbit

ObjectiveApplication BMSCs and original generation training constitutes a artificial bone graft ceramide radial defect materials to repair the rabbit, this paper discusses the BMSCs of allogeneic bone defect of study and the influence.MethodsApplication cell culture method from rabbit tibial BMSCs, in vitro isolating culture by osteogenesis induction. Will induce successful cells and HA compound after incubation, BMSCs/HA complexes formed the defects of the marrow, and graft. Trials are divided into 2 groups: group: for the application BMSCs/HA repair rabbit variants, the control group: for radial defect BMSCs/HA used autogenous radial defect repair rabbit, each group 12 only; Postoperative 2, 4, 8 weeks general observation, X-ray detection, HE dyeing,â… collagen type immunohistochemical stains, flow cytometric art detection achroacyte subgroup of artificial bone graft, observe autologous and allograft material in the process of immune rejection, and for this kind of rejection osteogenesis effect.ResultsThe original generation by BMSCs in the cultivation of in vitro, after induction BMSCs/osteoblast ceramide complex after eight weeks, 24 implant all survived rabbit, wound healingâ… period are. X-ray showed experimental and control group, after two to four weeks in a fiber in defect callus formation, 8 weeks of increased BMD, callus formation, two groups had no significant difference, Morphological observation and control group were no obvious lymphocytes, have apparent new bone formation, two groups had no significant difference, Flow cytometric analysis results show that CD4 +, CD8 + lymphocytes group-dividing situation no significant difference (P > 0.05).ConclusionsThe cultivation of in vitro original generation BMSCs osteogenesis induction, BMSCs autologous allogeneic transplant didn't causing obvious immune rejection, the viewing and allograft were not transplant, BMSC affect bone defect healing.