Expression Of Runx3 And Survivin In Different Pathological Status Of Gastric Mucosa And Correlative Research

Objective To study the expression of Runx3 and Survivin in chronic gastritis, intestinal metaplasia, atypical dysplasia and gastric carcinoma, inquire to clinical pathologic characteristic of differentiation, invasion and metastasis of gastric carcinoma, and investigate the expression of Helicobacter pylori(H.pylori), explore their action, relationship in the succession of gastric cancer.Methods The specimens of 115 gastric mucous lesion were collected by gastroscopes, and divided into 4 groups, according to gastroscopes situation and pathological outcome. The patients with chronic inflammation were 15, the cases of intestinal metaplasia were 30, the cases of atypical dysplasia were 30, and the cases of gastric carcinoma were 40. Distributional characteristics of age and sex of all case groups were analyzed by immunohistochemisty SP method; expression of Runx3 and Survivin in all case groups were detected by immunohistochemisty SP method and were taken statistical analysis; then expression of H.pylori were detected by C-Urea Breath Test and the relations with Runx3 and Survivin were taken statistical analvsis. The data was analyzed by SPSS 15.0.Results 1.No significant difference was calculated of age structure and gender structure in all case groups.2.The positive expression rates of Runx3 were 93.3%(chronic inflammation group),73.3%(intestinal metaplasia group). 50.0%(atypical dysplasia group),37.5%(gastric carcinoma group) respectively, showing a descending tendency.3.In gastric carcinoma group, the level of Runx3 was higher in high differentiation grade(71.4%), no lymph node metastasis(72.7%) andⅠ～Ⅱstage(58.8%) than in low differentiation grade (18.7%), lymph node metastasis(24.1%) andⅢ～Ⅳstage(26.1%) in gastric carcinoma group. Significant difference of positive expression rates of Runx3 were calculated among them statistically.4The positive expression rates of Survivin were 0.0%(chronic inflammation group),6.7%(intestinal metaplasia group),33.3%(atypical dysplasia group),62.5%(gastric carcinoma group) respectively, showing an ascending tendency.5.The level of Survivin expression was higher in low differentiation grade(81.3%) andⅢ～Ⅳstage (78.3%) than in high differentiation grade (35.7%) andⅠ～Ⅱstage(41.2%) in gastric carcinoma group, but no significant difference between lymph node metastasis(72.4%) and no lymph node metastasis (36.4%). 6.The level of runx3 expression was higher in negative group of H.pylori (74.5%) than in positive group of H.pylori(47.2%), but the level of Survivin eapression was higher in positive group of H.pylori(38.9%) than in negative group of H.pylori(20.9%).7. There was a negative correlation between the expression of Runx3 and Survivin in gastric carcinoma tissue (r=0.360).Conclusions 1.The defective of Runx3 expression and excessive expression of Survivin were involved with pathogenesis of gastric mucosa lesion and gastric carcinoma, they were involved with invasion and metastasis of gastric carcinoma. It demonstrated that Runx3 and Survivin could guide early detection and diagnosis of gastric carcinoma, and provide some ideas for the differentiation and stage of gastric carcinoma.2. H.pylori may affect the expression of Runx3 and Survivin. or they interacted with H.pylori in succession of gastric carcinoma. Combine detection of H.pylori, Runx3 and Survivin may contribute to the screening of high-risk population of gastric cancer.3. Runx3 and Survivin maybe play an antagonistic role of mutual inhibition in the gastric mucosal erosion and the succession of gastric cancer, they influence each other.4. In summary, Runx3 and Survivin were possible as a new target of early diagnosis and treatment of gastric cancer through genetic engineering technology, an entry point that block and reverse precancerous lesions.