Expression And Significance Of K17,7,10/13,8 And Ki-67 In Cervical Squamous Intraepithelial Lesion And Immature Squamous Metaplasia

Objective:Cervical cancer is the second most common cancer among women worldwide.Among them, the squamous cancer is more happened, cervical intraepithelial neoplasia is precancerous change of Cervical cancer.The difference between high risk chanciness and low risk chanciness is hard to distinguish. Keratins are a highly diverse family of intermediate filament and important markers of epithelial cell differentiation.. The composition of keratin pairs varies depending on cell type, differentiation status and environment,and a lot of researcher pay close attention to it for the auxiliary diagnosis of pathological changes. Our target was to explore the expression of keratins and Ki-67 in Cervical epithelial nonmalignant changes and tumorous changes, investigating the different of cell differentiation among Cervical epithelial changes and the function of keratins and Ki-67 as auxiliary diagnosis of pathologic diagnosis.We investigated the 2006 to 2009 formalin-fixed paraffinembedded cervical specimens:cervical punch biopsies, knife cone and loop electrosurgical excision procedure (LEEP) excision specimens with varying proportions of normal cervical squamous epithelium(n=10), immature squamous metaplasia(n=30), low-grade squamous Intraepithelial lesion(CINⅠ,n=30) and high-grade squamous intraepithelial lesion (CINⅡ=11,CINⅢ= 19) from the department of Pathology in the General Hospital in Tianj in Medical University. The expression of keratin 17,7,8,10/13 and Ki-67 were examined by immunohistochemical technique. The data was analyzed by SPSS software.Results:1.K17 was high expressed in cervical immature squamous metaplasia and low in normal cervical squamous epithelium,low-grade squamous intraepithelial lesion and high-grade squamous intraepithelial lesion.There was a significant difference between immature squamous metaplasia and high-grade squamous intraepithelial lesion P＜0.01) 2. There was a significant difference of the expression of K7 among each group, K7 expression in high-grade squamous intraepithelial lesion was higher than immature squamous metaplasia,low-grade squamous intraepithelial and normal cervical squamous epithelium,in the immature squamous metaplasia was higher than low-grade squamous intraepithelial, in the low-grade squamous intraepithelial was higher than normal cervical squamous epithelium (P<0.01)3.There was a significant difference of the expression of K10/13 among each group, K10/13 expression in high-grade squamous intraepithelial lesion was lower than immature squamous metaplasia,low-grade squamous intraepithelial and normal cervical squamous epithelium (P< 0.01), there was no statistic difference of K10/13 between low-grade squamous intraepithelial and normal cervical squamous epithelium (P＞0.05);4. There was no statistic difference of K8 among every group (P＞0.05);5. The expression of Ki-67 in high-grade squamous intraepithelial was higher than in others (P＜0.01)6. In normal cervical squamous epithelium,low-grade squamous intraepithelial and high-grade squamous intraepithelial, there were positive correlation between the expression of K7 and K10/13 (r=-0.427,P＜0.001)Conclusions:1.K17 was high expressed in immature squamous metaplasia,that means it maybe the "stress" protein when cervical inflammation,while had no significantly changed in malignant disease,so K17 was useful to distinguish them;2.K7 was up-regulated with the grade of disease,while K10/13 was converse.The change of them may means the cell differentiation disordered and may be the consequence of genetype mutation;3.There had no changed of K7,10/13 expression in cervical immature squamous metaplasia,the cell was well differentiated;4. Ki-67 was helpful to diagnose hyproliferation disease;5. Atypical immature metaplasia is a group of heterogenicity disease,with the correlation between immature squamous metaplasia and high-grade squamous Intraepithelial lesion,coexpression of K17,7,10/13 and Ki-67 can distinction the innocent change from malignant disease.