The Expression Of NGB And OPN In The Model Rat Of SSST And Investigated The Machanism Of Neuroprotective Effect Of EPO

Object:To establish animal model of superior sagittal sinus thrombosis and observation of neuroglobin and osteopontin in the rat on the expression of superior sagittal sinus thrombosis. As well as to research the PathoPhysiological and morphological change of the animal model's brain. To explore protective mechanism of EPO.Methods:72 healthy adult male SD rats were divided into normal group(n=6), sham operated group(n=18), model group(n=18) and EPO intervention group(n=18), and the all groups were divided again into three subgroups :6h(n=6),24h(n=6), 72h(n=6) the model group was that bridging vein were ligated injection of Activated Cephaloplastin Reagent in the posterior 1/3 SSS (n=18), EPO group was established by using intraperitoneal local intubation and injecting EPO(3000u/kg) two hours before surgery. The changes of neurological function classification ,HE staining ,cerebral water content, expression of NGB and OPN were measured in different groups.Results:1. Neuorlogieal deficit: it had no statistical significance between normal group and sham operation group(P>0.05); neurological function of the model group have notable deficit, neurological function of the EPO group have markedly improved than model group, it had statistical significance between all subgroups of model group and EPO group(P<0.05).2. HE staining: In normal group and sham operation group, nerve cell degenerationand necrosis was rarely. In model group, we found that neuron was raref-action, cell spaces was accreted and there existed a lot of degenerated and necrotic neurons, it mainly displayed cell body shrinkage, karyopycnosis and chromatospherite vanished. But EPO group had more survival nerve cells and the degree of injury was obviously lessen.3. Cerebral water content: it had no statistical significance between normal group and sham operation group(P>0.05);Cerebral water content in model group was more obvious than EPO group, it had statistical significance between all subgroups of model group and EPO group(P<0.05); Cerebral water content in model group was increased at 6 hours, achieved peak at 72hours. Cerebral water content in EPO group was less than model group.4. Expression of NGB in brain tissue: Each group's positive cells of NGB compared:it has no statistical significance between normal group and sham operation group(P>0.05)It had statistical significance among different subgroups in model group and EPO group (P<0.05): 6 hours compared with 24 hours, 6 hours compared with 72 hours , 24 hours compared with 72 hours, except 6 hours compared with 24 hours of EPO group have no statistical significance (P>0.05).5. Expression of OPN in brain tissue: Each group's positive cells of NGB compared:it had no statistical significance between normal group and sham operation group (P>0.05). Model group compared with EPO group, it had significant difference(p<0.05); It had statistical significance among different subgroup of model group and EPO group (p<0.05): 6 hours compared with 24 hours, 6 hours compared with 72 hours had significant difference (p<0.05).Conclusions:1. The rat model of SSST are feasible that established by ligated bridging veins and injection of Activated Cephaloplastin Reagent in the posterior 1/3 SSS.2. Expression of NGB and OPN was incresed in the state of brain ischemia and hypoxia. It may be pathogenesis of SSST. 3. The mechanism of neuroprotective effect of EPO possibly may be involved in increased the expression of NGB and OPN.