| Objectives To study therapeutic benefit and mechanism of intravenous administration of bone marrow mesenchymal stem cells after spinal cord injury in adult rats.Methods .Rat's acute SCI model was established with modified Allen's method。There are 48 in the sham-operated group and 96 rats in the injuried group. Then the injuried group was randomly divided into transplantation group and control group.There were 4 time points in each group,n=12 in each point. At 7days after SCI,5×l06 BMSCs (1ml) were injected into the transplantation group by tail vein and 1ml (PBS) were given to the control group by tail vein. BMSCs were labeled by GFP before transplantation。2.Functional out come meaurements using the Basso-Beatti-Bresneh-an Score were performed at the 1st d,3rd d,7th d and 14th d after transplantation.3.Light microscopy and electron microscopy were used to identify the morphology of the sections of injured spinal cord tissue at 14th d after transplantation. Laser scanning microscopy were used to identify migrati-on and survival BMSCs in vivo at the 3rd d and 7th d after transplantation.4.The expressions of Nogo-A and SYN were detected by immunohistoc-hemical method at the 1st d,3rd d,7th d and 14th d after transplantation.Results 1.neurological functions of transplantation group and control group all were recoveried in varying degrees,but neurological function in the transplantation group was higher than it in the control group (P＜0.05).2.GFP staining positive cells were obviously gathered and survived in the injury zone and its surrounding area at the transplantation group.3.the expressions of Nogo-A in the transplantation group was down-regulated more significantly than the control group at the 3rd d,7th d,14th d after transplantation.(P<0.05)4.the expressions of SYN in the transplantation group was higher significantly than the control group at the 3rd d,7th d,14th d after transplantation.(P<0.05)Conclusion1.BMSCs transplanted by vein can penetrate through the blood-brain barrier, to spinal cord injury, and regional occurred in vitro migrating live, differentiation promote rat acute spinal cord injury nerve function recovery o2.BMSCs transplantation in the treatment of acute spinal cord injury can inhibit the damaged local Nogo-A protein expression, thus reducing glial scar formation, promote the regeneration of axons.3.Transplantation in the treatment of acute spinal cord injury BMSCs to local SYN expressing increasing, promote synaptic function recovery.
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