Expression Of Survivin In Single Nuclear Cell Of Rheumatism Arthritis Patient Circumference Blood And Its Significance

Background and Objective:Rheumatoid arthritis (rheumatiod arthritis, RA) is take polyarticular exhausts oneself as main performance own immunological disease, its main pathology characteristic is the synovial cell (mainly to become desmoid synovial cell) the unusual proliferation, the lining level accumulation, the inflammatory cell infiltration (macrophage, the T cell and so on), forms class the rheumatic pannus and corrodes the cartilage and the bone, finally causes the joint to destroy, although in RA the most prominent immunity pathology event occurs in the joint spot and the synovial membrane, not only but the immunity exceptionally and seeps out limited to the synovial membrane lymphocyte, but also includes in the circulation the majority of T lymphocyte. The medicine is the RA important treatment method, and often for many kinds of medicines long-term, union application. But bore the medicine the appearance actually to hinder the development which kind of rheumatism arthritis treated, the multi-medicines bore the medicine (Multiple Drug Resisitance, MRD) the occurrence was the serious influence treatment effect, but the RA patient had the MDR mechanism clearly still not. Recent years the massive research discovery might examine in the majority RA patient's synovial cell and the synovial fluid anti-perishes protein Survivin exceptionally to exist weakly. Survivin takes perishes weakly suppresses the protein (inhibitor of apoptosis protein, IAP) one of family members, was discovered until now strongest perished weakly the inhibiting factor, was also the present molecular weight smallest IAP protein. It has the suppressor cell to perish weakly, promotion cell multiplication function, but also participates in the adjustment cell mitosis as well as the vascularization. Some research indicated that Survivin exceptionally expressed participated in the RA occurrence, but it with difficult RA existence what kind of relations still not bright, this experiment used the immunocytochemistry examination class rheumatism arthritis patient circumference blood single nuclear cell (Peripheral blood mononoclear Cell, PBMC) the Survivin expression situation, discussed it to bear the medicine at the difficult class rheumatism arthritis the formation mechanism, to seek bears the medicine the reversal machine-made and the safe effective reversal medicinal preparation provides the full theory basis and the new mentality.Method:According to enters the group standard collection class rheumatism arthritis patient to divide into RA to start has not governed the group, RA to treat the effective group, the RA difficult group* take the healthy volunteer as the normal control group, extracts circumference venous blood 10ml separately and separates the single nuclear cell, the application immunocytochemistry technology examines in each group of specimens the Survivin protein expression situation.Results:The kind of rheumatism arthritis first diagnosis has not governed in the group circumference blood single nuclear cell the Survivin masculine gender expression rate to elevate obviously compares with the normal control group has the significance difference, (χ2=29.59, P<0.01;t=8.34, P< 0.01). RA treats in the effective group circumference blood single nuclear cell the Survivin masculine gender expression rate to have slightly elevates, compares the non-significance difference with the normal control group (t=1.25, P> 0.05), RA difficult to govern the group survivin masculine gender expression rate to elevate obviously, with normal control group(χ2=26.53, P<0.01;t=8.65,P<0.01), with Treats the effective group (χ2=24.35, P<0.01; t=7.12, P<0.01) compares wih them has the significance difference. Has non-significance difference comparing with the RA to start has not governed the group (X2=0.014, P>0.05;t=0.15, P>0.05).Conclusion:In rheumatism arthritis patient circumference blood single nuclear cell the Survivin expression elevates obviously, prompts that Survivin has influential role in the occurrence and development of rheumatism arthritis.in the Kind of rheumatism arthritis difficult to govern the Survivin expression elevate obviously, prompts the Survivin possible participation class rheumatism arthritis multi-medicines to bear the medicine the formation to live.