Effect Of Intensities Of Electroacupuncture On C-reactive Protein And Interleukin-6 In Adipose Tissues From High Fat Diet-induced Obese Rats

ObjectiveTo investigate the effect and mechanism by electroacupuncture on C-reactive protein (CRP) and interleukin-6 (IL-6) in adipose intensities of electroacupuncture on weight loss.MethodsMale SD rats were fed with high fat diet to induce obesity,Obese rats were randomly devided into model group with high fat diet only(MG, n=10), electroacupuncture groups with high fat diet fed rats stimulated by either 2v (2V EA,n=10) or 1v of electroacupuncture (1V EA,n=10),and set up control group(CG, n=10)simultaneously.Electroacupuncture (1O Hz, 2V EA: 2V, 1V EA:1V) was applied to acupuncture points"Zu san li"and"San yin jiao"for 15min everyday and for 14 days. Lee's index in each group were detected. Total cholesterol(TC), triglyceride(TG), high- density 1ipoprotein (HDL) and low-density 1ipoprotein(TG)were measured.The expression of CRP mRNA and IL-6 mRNA in epididymis adipose tissue was detected by reverse transcriptase polymerase chain reaction(RT- PCR). The contents of CRP in epididymis adipose tissue were detected by ELISA.The expression of IL-6 in epididymis adipose tissue was detected by Western blotting.ResultsAfter EA,Lee's index and serum content of TC,TG and LDLfrom two EA groups were significantly decreased while the content of HDLwas increased compared with the model group(P<0.01).expression of CRP,IL-6 from two EA groups were significantly decreased compared with the model group(P<0.01,P<0.05),and obvious change was observed between the two EA groups (P<0.01,P