| Background: Breast cancer is one of common malignant tumors in women, the malignant degree and prognosis vary widely. So many of today's treatment options for breast cancer tend to individualized treatment, and the implementation of individual treatment depend on the premise that making accurate prognosis of patients with different breast cancer. Therefore, the prognosis of breast cancer is a major issue which has been gat much attention.CTSD gene as cathepsin D gene, coding for the formation of functional protein Cathepsin D. in recent years, a lot of information show the relationship of overexpression in breast cancer with invasion, metastasis and prognosis,.the expression of Cathepsin D-positive breast cancer patients relapse early and easy transfer. Early expression pattern of the array of methods used in 45 cases of breast cancer detection and matching with the expression of metastasis-related genes and found that CTSD gene expression in metastatic breast cancer group increased.Gene methylation as an important way of epigenetic modification, it can affect both the promoter and transcription binding elements and affect the formation of chromatin loop to change gene expression and regulation . Nancy B found enhancer can bind to promoter region by the helpe of estrogen response element in MCF-7 cell line , and thus increase gene expression . whether is it existence of such regulation In breast cancer? To further explore the CTSD gene upregulated mechanism in breast cancer, the paper examined methylation of the breast cancer CTSD gene enhancer, to study the relationship of epigenetic modification of CTSD gene and breast cancer metastasis.Objective: To detect genetic and epigenetic changes of breast cancer CTSD gene enhancer, study its relationship with breast cancer metastasis. Methods: The sample selected from the Tumor Hospital of Jiangsu Province , breast cancer surgery 2002-2004 paraffin tissue samples. one group of 75 patients with metastasis, the other 77 patients with median follow-up 5-year no metastasis been found, access to medical record information, access to the results of all the unified entry unified coding, EPIDATA2002 entered into the computer software to establish a database, statistical analysis. Application of PCR amplified DNA sequence analysis of the United CTSD gene Ehancer detect genetic changes; applications combined PCR restriction enzyme digestion methylation detection CTSD gene amplification techniques Ehancer epigenetic alterations; application of bioinformatics methods pathogenic mechanisms, all Statistical analysis were carried out in the STATA7.0.Results:1. CTSD gene Ehancer genetic study1.1 152 cases of breast cancer specimens by direct sequencing PCR products, eliminate interference, found no significant genetic changes in CTSD gene Ehancer. That affect their prognosis of breast cancer genetic mutation is not achieved by enhancing the child's.2. CTSD gene Ehancer epigenetic alterations in breast cancer prognosis2.1 Contrasting the corresponding adjacent tissues (methylation rate of 94.7%),the demethylation rate was 51.9% in patients with 4 years median follow up of breast cancer without metastasis.the difference was statistically significant (P <0.05). Metastatic breast cancer tissue enhancer demethylation rate was 85.3%, compared with no metastasis was significantly (P <0.05)2.2 Tips CTSD gene enhancer demethylation may be involved in breast cancer, metastasis, in part prompted the patient's prognosis.3. The methods of methylation studyConclusion:the regulation of CTSD gene enhancer function got no impact from genetic mutations, the demethylation rate of breast cancer metastasis, is expected to be a prognostic indicator of breast cancer. |
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