Experiment Study On The Effect Of Resistin On Proliferation, Invasion And Metastasis In Human Cholangiocarcinoma Cells In Vitro

BackgroundVarious epidemiologic studies have shown that obesity is associated with primary cholangiocarcinoma. Resistin, the key player in the regulation of energy balance, also acts as a growth factor on certain organs in both normal and disease states. However, a direct role for resistin in cholangiocarcinoma has not been shown. In this study, we firstly explored the potential biological effects of resistin and the probable mechanisms underlying its action in cholangiocarcinoma cells.ObjectiveTo investigate the effects of recombinant human resistin (RHR) on the proliferation, invasion and migration abilities in human cholangiocarcinoma QBC939 cells,and approach the possible mechanisms.MethodsThe human cholangiocarcinoma QBC939 cells cultured in vitro was treated with different concentrations resistin. The proliferation of the cells was measured by MTT Assay; transwell chamber assay were performed to determine the effect of resistin on the migration and invasion of QBC939 cells. The expression of matrix metalloproteinase-2 (MMP-2) protein of QBC939 cells were detected by Western Blot.ResultsRHR significantly promoted the proliferation of QBC939 cells when the concentration of RHR was higher than 10 ng/ml(P<0.01),the effect was dose-dependent in some certain by MTT method; after treated with 10, 50,100ng/ml RHR for 24 h, the capacity of invasion and migration of QBC939 cells were significantly stronger in RHR group than in the control group(P<0.01); a significant up-regulation of the MMP-2 protein expression was observed in QBC939 cells treated with resistin for 24 h.ConclusionResistin could promote the proliferation, invasion and migration abilities of the human cholangiocarcinoma QBC939 cells in vitro, and the possible mechanism may be related with up-regulation of MMP-2 protein level.