Study On The Role And Mechanism Of ECM1in Cholangiocarcinoma Migration

Although the expression of extracellular matrix protein-1(ECM1) has beendocumented in several tumour models, the function of ECM1has remained unclear. Inthis study, expression of ECM1was detected by real time PCR andimmunohistochemistry. The role and mechanism of ECM1overexpression incholangiocarcinoma (CCA) cells were assessed by wound-healing, matrigel invasionassay and Western blotting. Expression of ECM1was significantly elevated in CCAtissues than that in adjacent noncancerous, cholangitis and normal bile duct tissues. Itsoverexpression was associated with poor differentiation, lymph node metastasis, poorprognosis, and the level of CA199, MMP-9, estrogen receptor. Knockdown of ECM1suppressed migration and invasion of CCA cells. Using PI3K or IKK inhibitorreduced the level of phospho-Akt or phospho-IκBα as well as ECM1. Taken together,overexpression of ECM1may contribute to CCA initiation and progression throughpromoting migration and invasion of CCA cells, its overexpression was associatedwith Akt/NF-κB signaling axis.