Effect Of Resveratrol On Oxidative Stress And The Expression Of P27,p21 Protein In Rat Mesangial Cells Under High Glucose

Objective To investigate the effect of Resveratrol on oxidative stressand the expression of p27,p21 protein in rat renal mesangial cells under high glucose.Methods Cultured rat renal mesangial cells (RMC) were divided into four groups:normal glucose (5.6 mmol/L), normal glucose plus Resveratrol (20μmol/L), highglucose (30 mmol/L), high glucose plus Resveratrol at 20μmol/L. After treatment for72 h, the levels of malondialdehyde (MDA) in cell supernatant were assayed usingchromatometry. The levels of reactive oxygen species (ROS) were detected by flowcytometry. The degree of RMC hypertrophy was estimated through counting the ratioof the RMC protein content to the number. The protein levels of p21and p27 weredetected by Western blotting. Results (1) As compared to the NC group, exposure ofrat renal mesangial cells to D-glucose at concentrations of 30 mmol/L caused asignificant increase in the levels of MDA, ROS (P<0.01). The addition of Resveratrolattenuated high glucose- enhanced levels of MDA, ROS (P<0.05). (2) As comparedto the normal cells, exposure of rat renal mesangial cells to D-glucose atconcentrations of 30 mmol/L caused RMC hypertrophy and a significant increase inthe protein levels of p27 (P <0.01), the protein levels of p21 also increased (P <0.05);The addition of Resveratrol attenuated RMC hypertrophy and high glucose-enhancedprotein levels of p21 and p27 (P <0.05). But no notable difference at the proteinlevels of p21 and p27 was found between the Res and NC group (P>0.05).Conclusion Resveratrol may attenuate RMC hypertrophy through reversing highglucose up-regulated oxidative stress and the expression of p21 and p27 in RMC.