Effect Of GnRH-a On The Expression Of Aroma Tase And Cyclooxygenase-2 And IL-1β　in Endometr Iotic Rats

Background: Endometriosis is a hormonal dependent,polygenic multi- factorial and hemorrhagic disease that affects 10% to 15% of women of reprodutive age. This disease can bring chonic pelvic pain and infertility for women,and seriously affect the quality of life of women. Although EM is a benign disease, but it is the performance characteristics of plant growth and metastasis, making it difficult to treat disease. Therefore, The problEMS which need to be solved in EMs research is studying the pathogenesis of EMs and to find effective treatment. Gonadotropin-releasing hormone agonist (GnRH-a) as effective drugs for treatment of endometriosis have been widely used clinically, but the adverse reactions have not found the exact ways of improvement in the course of treatment. There is growing evidence that the organization of target local synthesis of estrogen, disease incidence of different pathophysiological events plays an important role, Aromatase is acytochrome P450 enzyme which catalyzes the rate-limiting step in estrogen biosynthesis.Cyclooxygenase-2 catalyzes the biosynthesis of PGE2 which is found to be the most potent known inducer of aromatase activity, And there are reports that IL-1βcan induce COX - 2 expression. Surgically transplanted autologous uterine tissues to ectopic sites beside the uterines in rats were used as animal models to study endometriosis, and study the influence of GnRH - a for P450arom, COX - 2 in and IL-1βin EMs. In order to investigate P450arom, COX - 2 and IL-1βamong the three relationships, and the significance of the EMs occurrence and development.Methods: Surgically transplanted autologous uterine tissues to ectopic sites beside the uterines in rats were used as animal models to study endometriosis ,after 4 weeks, The models were then divided into five groups: Model control group, GnRH - a high, medium and low dose group and mifepristone influences group. Four weeks after treatment, to observe the size of each model lesions and HE dyeing observation endometrial growth pattern. PV immunohistochemical method was used to detect p450arom and COX-2 protein expression in the ectopic endometrium of rats with endometriosis. Reverse transcript-polymerase chain reaction(RT-PCR) was used to detect P450arom mRNA and COX-2 mRNA expression in the ectopic endometrium of rats with endometriosis . ELISA method was used to detect IL-1βcontent in the celiac fluid of rats with endometriosis.Results:1 Endometriosis card be successfully created in the rat model . Druging four weeks later, to observe each index., each group lesion volume have obvious difference (P < 0.05),and it have obvious difference compared with before druging,too.2 HE staining, ectopic lesions under the microscope for histological observation,the lesion is vesicle membrane from inside to outside, muscularis and serosa layers, showing that ectopic endometrial hyperplasia, endometrial epithelial cells in long columnar, some hyperplasia was pseudostratified structure.3 RT-PCR and detected by SABC immunohistochemical model of ectopic lesions in each group, both P450arom and eutopic expression of COX-2, stronger than the eutopic endometrium in ectopic endometrium (P <0.05); the blank control groupstronger than the drug group were ectopic, eutopic endometrium, between two statistically significant difference (P <0.05); the drug group may inhibit ectopic, eutopic endometrial P450arom and COX-2 expression dose of GnRH-a in the strongest effect, with the other drugs difference between two groups was statistically significant (P <0.05).4 ABC-ELISA, each group in both peritoneal fluid and serum IL-1βexpression in the control group were stronger than the drug group, the difference between two statistically significant. (P <0.05); GnRH-a in the strongest dose group, with the other drugs difference between two groups was statistically significant (P <0.05). 5 Linear correlation analysis P450arom, COX-2 both in the EMs in the role of correlation.Conclusion:1 Non-estrus combination of preoperative and postoperative added in the amount of estrogen successful rat EM model, the modeling success rate 76.9% (40/52).2 GnRH-a have good resistance to EMs estrogen generate role, possibly through fall down the expression of P450arom, COX - 2 and IL - 1 beta in ectopic endometrium organization, block positive feedback loop of estrogen synthesis. achieve remedial endometriosis card purpose.