Studies On Preparation Technique Of Effective Fraction To Cure Chronic Pelvic Inflammatory Disease And Therapeutic Basis Of Smilax China L.

Smilax china L. which was also named as Smilax china L. is liliaceous plant chinaroot greenbrier Smilax China L's dry roots. With the efficacies of benefit to dampness and taking turbidity out,dispelling endogenous wind and paralysis,detoxification and scattered stagnate, it has been widely used in gynecology chronic inflammation,adnexitis inflammation and other diseases. Four hundred years ago, its usage as medicine was recorded, since the 1960s, its total extract preparation has been widely used in gynecology chronic inflammation,adnexitis inflammation and regulation immune, promoting blood circulation to remove blood stasis, anti-tumor and other disease.In recent years, there were a lot of study on chemical components of Smilax china L., it's very complex, mainly contains saponins, flavonoids, stilbenes, tannins, amino acids and so on, the saponins and flavonoids compounds are as the main anti-inflammatory component. The ultraviolet spectrophotometry was used in the subject, by the choice of reagent, sample pretreatment screening to establish the total saponins and total flavonoids determination method, and conducted a series of methodology investigation, it shows that the content determination method is feasible, and it found a deep base for Smilax china L. quality control and further research and development, it was used the established methods to determine the collection of eleven batches of Smilax china L. medicinal materialsl total saponins and flavonoids content. Considering the polarities of steroidal saponins and flavonoids and the compact cell wall structure, we choose the heating backflow (water and alcohol), enzymatic hydrolysis, permeability Percolation and supercritical fluid extraction five different extraction methods to study the extraction process on Smilax china L. We choose the orthogonal experiment method to optimize the extraction process of Smilax china L, using macroporous resin to pure optimization, screened resin types and types, and finalized that HPD-300 purification with Smilax china L coarse extraction, and the various parameters were optimized in purification process, we found the best extraction and purification process method of effectively parts extraction. through the primary efficacy experiment, we fill the Smilax china L. different extract compositions mixed bacteria to rats, it caused the rats sicken chronic pelvic inflammation, it was confirmed that the compositions was the effective parts of the Smilax china L. on inhibiting uterine tumefaction, it's reached five kinds of new drug standards. Eventually, the total saponins and the flavonoids content can reach more than 60%, the process were stability and feasible, suitable for industrial production.Adopt LC-MS identify extracts of chromatographic peak, of Smilax china L.'s different process to five identified flavonoids, two saponins, one stilbene. And use organic solvent extraction technology, silica gel column chromatography, and the gel column chromatography of polyamide column chromatography technology, Smilax china L. anti-inflammatory effective parts of the chemical composition of separation, and system using UV, MS,'H-NMR,13C-NMR. DEPT.HMBC, HSQC, such modern spectrum technology, seven compounds were isolated from, appraisal get five, preliminary illuminates the Smilax china L. anti-inflammatory effect of a substance basis...