| Objective 1,to establish a simple, reproducible, high success rate of the rat Portacaval transposition and portal vein arterialization models.2 to research Portacaval transposition and portal vein arterialization in the liver of normal rats of ischemia reperfusion injury.Method 1, Cuff using allograft materials and methods of vascular rat Portacaval transposition model. Removal of the left kidney, left renal vein at the end of the installation of a cuff, the left renal vein and the portal vein stump by cuff connected, affixed above the left renal vein ligation and cut the inferior vena cava, the inferior vena cava and sleeve by allogeneic blood Set of connections with the superior mesenteric vein was observed in rats 2 months after the weight, biochemical parameters, liver and liver pathological changes of magnetic resonance imaging;2, Cuff using allograft materials and methods of vascular rat model of portal vein arterialization. Removal of the left kidney, the left renal artery and portal vein stump material connected by allogeneic blood vessels, the left renal vein by cuff connected with the superior mesenteric vein was observed in rats 2 months after the body weight, biochemical parameters, liver magnetic resonance imaging And liver pathological changes;3,Transposition of portal vein access cavity model, the portal vein and artery model as a control group of normal rats 10, blocking the door in the first 25 minutes of liver were observed in rats after 6 h, 12 h, 1 d, 3 d Weight, alanine aminotransferase, aspartate aminotransferase, liver pathological changes4, Transposition of portal vein access cavity model, the portal vein and artery model of the 10 normal rats, blocking the door in the first 25 minutes of liver were observed in rats after 6 h of serum NO and plasma ET-1 and liver iNOS and ET -1 immunohistochemistryResults 1, rat vena portal vein and artery of translocation after short and long term survival of good, after 24 h to 2 month survival rate were 95% (19/20). After 2 weeks of liver blood flow magnetic resonance imaging showed. 2 months after groups of rats albumin, alanine aminotransferase, alkaline phosphatase and total bilirubin, weight and other indicators showed no significant difference (P> 0.05). 2 months after the pathological examination were normal2, rat portal vein lumen translocation and hepatic artery of the door in the first 25 minutes after occlusion, compared with the control group, two groups of rats after 6 h, 12 h, 1 d, 3 d body weight, alanine Aminotransferase, aspartate aminotransferase index showed no significant difference (P> 0.05), liver pathology test results were normal3, rat portal vein lumen translocation and hepatic artery of the first 25 minutes after blocking the door and found that 6 h of serum NO, plasma ET-1 group and the chamber door translocation PVA group than control group higher P <0.05 was statistically significant, 6 h immunohistochemistry showed that PVA group the expression of iNOS and ET-1 was higher Portacaval transposition group the expression of iNOS and ET-1 was significantly higher and arteries of the group, Difference was significant (P <0.05)Conclusion 1, the use of allograft vascular cuff method with rat portal vein lumen translocation and PVA model is reliable, and its good reproducibility, high success rate;2, vena portal vein transposition in rats with portal vein arterialization after ischemia-reperfusion injury in liver of the recent non-discriminatory;3, portal vein arterialization of portal vein in rats than the cavity shifted more tolerance in rats ischemia and reperfusion injury.
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