Nonlinear Spectral Imaging Of Capillary Hemangioma Of Children Based On Two-photon Excited Fluorescence And Second-harmonic Generation

Objective To investigate the potential ability of nonlinear spectral imaging method based on combination of two-photon excited fluorescence (TPEF) and second harmonic generation signals (SHG) for Non-destructive detection and monitoring of the pathophysiology of capillary hemangioma among children and for tracking the process of development of them as well as for assessment treatment responses in clinic. Methods Selected 58 cases of skin capillary hemangioma of children ,pathology proved that 26 cases (2-12 months) in proliferated stage, 16 cases (2-5 years) in degradation stage , 16 cases (6-11 years) in completed stage. All of the tissue section samples of 30 lm thickness were analyzed by the use of a nonlinear spectral imaging system, normal skin in the control group. Use nonlinear spectral imaging system to image and analysis the spectral of samples. Results There are significant differences between proliferated stage of capillary hemangioma tissue and normal skin in terms of their morphological structure and the spectral characteristics of collagen and elastic fibers. The difference are significantly reduced in degradation stage and are no significant difference in the completed stage. In proliferated stage, degradation stage the completed stage of capillary hemangioma tissue, the intensity of collagen SHG signal and depth dependent decay constant of collagen decreases compared with the corresponding normal skin, diameter of elastic fibers in the lesions increased gradually until no difference with normal skin. Conclusions All the measurement data and nonlinear spectral images demonstrated that there are many differences between capillary hemangioma tissue which in different stages and normal skin, the differences can distinguish them as important indicators. The nonlinear spectral imaging based on SHG imaging and TPEF imaging will be used for non-destructive detection and real-time monitoring in the pathophysiological process of capillary hemangioma, tracking the course of the development process, as well as become a powerful tool for guiding treatment and evaluating treatment effects in clinic.