Experiment Of The Protective Effect Of Liucha Extractive On Liver In Non-Alcoholic Fatty Liver Disease Rats

Objective:Observing the effect of Liucha extractive on liver functional lesion, antioxidant capacity and morphological damage in non-alcoholic fatty liver disease rats, to investigate the relationship between Liucha extractive and the gene expression of key enzymes involving in lipid metabolism, exploring the way by which peroxide was cleaned up meanwhile, thereby argument the possible mechanism what the liver of non-alcoholic fatty liver disease rats were protected by Liucha extractive, and offering a theoretical basis for the efficacy and security of the clinical application.Methods:Non-alcoholic fatty liver disease model of SD rats were established by high-fat diet feeding for 6 weeks.After divided randomly into 4 groups,the rats were intragastrically given normal sodium, Liucha extractive of low,medium,and high-dosage respectively for 8 weeks before killed. Meantime, SD rats of normal control group were ordinary-diet fed for 6 weeks, and killed after having been given normal sodium intragastrically for 8 weeks.Liver function parameters ALT, AST and the contents of TG, CHOL, LDL, HDL in liver homogenate or serum were examined.The pathological section of hepatic tissue was made and morphological change was observed under a light microscope. The relative mRNA expression of PPARa, ACO, LCAD, CYP4A1, which play a important role in liver lipid metabolism, were measured by RT-PCR and SYBR Green real time PCR. Expression of antioxidant enzymes CAT, GSH-Px mRNA were detectived using the same methods. The expression of key enzyme, PPARa protein were detected by Western Blot.Results:Compared with non-alcoholic fatty liver disease model group, ALT level of Liucha extractive low, medium dose groups decreased, the difference was statistically significant(P<0.05). AST level in low, medium dose groups decreased (P<0.05 in low dose group, P<0.01 in medium dose group). Content of CHOL and TG of liver homogenate in Liucha extractive medium dose group decreased (P<0.01, P<0.05 respectively). CHOL content of Low and high dose groups decreased (P<0.05). Serum CHOL, TG level of Liucha extractive low, medium, high dose groups dropped, HDL level raised, the difference was statistically significant (P<0.05). Pathological section of hepatic tissue shown liver cells fatty change reduced, cellular edema and necrosis improved in Liucha extractive groups to different degree. PCR results shown expression of PPARa mRNA was increased in Liucha extractive medium, high dosage groups, the difference was statistically significant (P<0.01). Expression of ACO mRNA was strengthened (P<0.05 in low dosage group, P<0.01 in medium and high dosage groups). LCAD mRNA was increased (P<0.05 in low dosage group, P<0.01 in medium dosage group). Expression of CYP4A1 was up-regulated, the difference was statistically significant (P<0.05 in medium dosage group, P<0.01 in high dosage group). Compared with the model group, expression of antioxidant enzyme CAT mRNA was strengthened, the difference was statistically significant (P<0.05 in low and high dosage groups, P<0.01 in medium dosage group). Expression of GSH-Px mRNA was up-regulated, the difference was statistically significant (P<0.05 in low dosage group, P<0.01 in medium dosage group).Western Blot shown the protein expression of PPARa, the core enzyme, was increased corresponding to the dosage of Liucha extractive.Conclusion:Liucha extractive could repair tissue damage and functional lesion in liver of non-alcoholic fatty liver disease rats, promote the velocity of lipid metabolism and antioxidant capacity. The protective effect was realized by adjusting the expression of PPARa, the core enzyme which involved in liver lipid metabolism and its target enzymes ACO, LCAD, CYP4A1 and CAT, GSH-Px in gene or protein level.