| BackgroundThe root cause of acute leukemia relapse is in remission patients still exists traces of leukemia cells, namely Minimal Resi-dual Disease(MRD) Qinghao Biejia Tang is recognized as an effective prescription, in the Yin deficiency internal hot and evil poison volts leave Yin points; in the clinical treatment for leukemia has made a good effect, even in recent years many studies have found that Qinghao Biejia Tang in vitro anti-tumor effect, and a small amount of literature of Qinghao Biejia Tang in vitro apoptosis of leukemia cells, and this new use of its anti-leukemia provides an experimental basis. The modern growing problem of drug resistance of leukemia, the existing anti-leukemia drug has been difficult to cope, to develop a new anti-leukemia drug has a huge cost, with the WenBing as clinical applications are years, can Jiawei Qinghao Biejia Tang developed as anti-leukemia drug, will save a lot of time and money to achieve a multiplier effect.ObjectiveStudy of Jiawei Qinghao Biejia Tang in acute myeloid cells leukemia Patients of bone marrow cells CD antigen expression effect, so as to Jiawei Qinghao Biejia Tang treatment of acute leukemia basis for further clinical applications.MethodsSelected 60 patients with newly diagnosed or relapsed acute myeloid cells leukemia Patients, Divided into the control group and the observation group, After therapy, two groups of patients were gathering around the before and after the treatment of bone marrow tissue, For experimental observation Flow cytometric analysis determination patients before and after treatment of bone marrow cells CD13,CD33 expression rate; Take heparin anticoagulation marrow 20μl, add OptiLyseC hemolysin 500μl, Room-temperature 10 minutes, add sheet resistance to room temperature for 10 minutes, PBS washing 2 times, Adjusting individual nucleus cell number 100 million/1ml computer detection,, In order to investigate the Jiawei Qinghao Biejia Tang flavored of acute myeloid cells leukemia patients remission of bone marrow cells CD antigen expression effect, Research The role of the Jiawei Qinghao Biejia Tang in treatment for leukemia.ResultThe results showed that Jiawei Qinghao Biejia Tang acts on primary human acute leukemia cells, Taking Jiawei Qinghao Biejia Tang observation group before and after the treatment of bone marrow cells CD13, CD33 expression rate decreased, in which the control group middle and observation group were statistically significant.ConclusionResearch suggests to remove poison through poison centralizer for legislation bases of Jiawei Qinghao Biejia Tang complete remission after acute myeloid cells leukemia patients before and after the treatment of bone marrow cells CD33 expression rate CD13, had a positive effect. |