The Biological Significance And The Related Study Of Androgen Receptor And PAK6 In Benign Prostate Hyperplasia And Prostate Cancer

Objective:We intended to detect the expressions of Androgen Receptor(AR) and p21-activated kinase 6 (PAK6) in prostate cancer(PCa) and benign prostate hyperplasia(BPH). and to discuss their relationship with clinicopathological features in Prostate cancer.Moreover, we analyze the probably related mechanism between two protein in Prostate cancer.With these results, We hoped to be helpful for early diagnosis, gene therapy and prognosis evaluation of Prostate cancer.Material and Method:All samples in our research were collected from the First Affiliate HosPital of Jinan University from February 2009 to March 2011.All patients were not enforced radiotherapy, chemotherapy and hormone therapy before operation and were diagnosed by Haematoxylin-eosin dyeing of histopathology. All samples were collected from patients of transurethral resection or radical excision, in which there were 50 cases of prostate cancer,40 cases of benign prostate hyperplasia.We used SP immunohistochemistry method to detect the expressions of AR and PAK6 proteins. All data were processed by SPSS version 13.0 analysis software. Chi square tests was used to compare the ratios of which Androgen Receptor and PAK6 protein were respectively detected in two groups. By using Spearman rank correlation analysis, the correlation between index of express magnitude in group.Result:1.Androgen Receptor protein were respectively detected in 87.5%(18/20)and 56.0%(22/28)of Benign prostate hyperplasia and Prostate cancer. AR protein in Benign prostate hyperplasia is significantly higher than that of Prostate cancer(P<0.01).2.The positive rate of AR protein in 40 cases of Pca with different Gleason grade respectively are:poorly differentiated group 26.7%(4/15), middling differentiated group 56.3%(7/16), well differentiated group 89.5%(17/19), the positive rate is significantly difference in total(P<0.01). AR protein in well differentiated group is significantly higher than that of middling differentiated group(P<0.01), and the positive rate of AR protein in well differentiated group is significantly higher in poorly differentiated group(P<0.01). There are no significant difference between poorly differentiated group and middling differentiated group in PCa(P>0.05).3.The positive rate of AR protein was higher in the group 65.0%(26/40) without metastasis than that with metastasis20.0%(2/10) in PCa(P<0.05).4.In PCa,the expression degrees of AR protein has significantly negative correlation with the differentiation degrees(P<0.05).5.PAK6 is respectively detected in 76.0%(38/50) and 40.0%(16/40)of Prostate cancer and Benign prostate hyperplasia. The positive rate in Pca is significantly higher than that in the group of BPH(P<0.01).6.The positive rate of PAK6 protein in 50 cases of PCa with different Gleason grade respectively are:well differentiated group 53.8%(7/13), middling differentiated group71.4%(10/14), poorly differentiated group 91.3%(21/23), the positive rate is significantly difference in population(P<0.05). PAK6 protein in poorly differentiated group is significantly higher than that of well differentiated group(P<0.05),There are no significant difference between middling differentiated group and poorly differentiated group or middling differentiated group and well differentiated group in PCa(P>0.05).7.The positive rate of PAK6 protein was higher in the group 72.5%(29/40) without metastasis than that with metastasis 90.0%(9/10) in PCa(P<0.05).8.1n PCa, the expression degrees of PAK6 protein has significantly positive correlation with the differentiation degrees(P<0.05).9.In PCa, the expression degrees of PAK6 protein has significantly positive correlation with the expression degrees of AR protein (P<0.05).Conclusions:1.The positive rate of AR protein in Benign prostate hyperplasia is higher than Prostate cancer, which suggest that AR expressing may be related to the genesis and progress of Prostate cancer, it may play an important role in the early stage of Prostate cancer; AR may have important value in early stage diagnosis of Prostate cancer and Benign prostate hyperplasia.2.The positive rate of AR protein is higher in metastasis group than that of metastasis group in PCa, which suggest that AR expressing may be related to infiltration and metastasis of Prostate cancer, and be related to the progression and malignant biological behavior; It can be a reference factor in prognosis evaluation. 3.The positive rate of AR protein in well differentiated group is significantly higher that of poorly differentiated group in PCa, which suggest AR may be played an important role on inhibiting infiltration and metastasis and may promote differentiated of cell. It can be a important reference factor in prognosis evaluation.4.The positive rate of PAK6 in PCa is significantly higher than that Benign prostate hyperplasia, the positive rate of PAK6 in poorly differentiated group is significantly higher than that of well differentiated group in PCa, and the positive rate of PAK6 in metastasis group is significantly higher that of without metastasis group in PCa, which suggests that PAK6 may play an important role in the genesis and development of Prostate cancer. It can have an interaction with AR as a proteinum, which can subdue the transcriptional activation of AR. It may suggest that PAK6 could be the specific marker in early diagnosis of prostate cancer and estimate therapeutic efficacy.5.AR play an important role in the genesis and development of Prostate cancer, PAK6 can have an interaction with AR as a proteinum, which can subdue the transcriptional activation of AR. The combination of these two tumor markers contribute to genesis, development and metastasis in Prostate cancer tumor cells. AR and PAK6 may be the specific markers in early diagnosis of Prostate cancer.