| On the basis of stable and reliable fluid percussing traumatic brain injury(TBI) models, we studied the therapeutic effects of Yunnan Baiyao on the model rats by testing Neurological Severity Score(NSS),cerebral edema, apoptosis index and related Caspase-3 gene expression.Part One:The foundation of moderate or severe TBI model and the effect of Yunnan Baiyao on NSS and cerebral edema in TBI model rats.Objective:On the basis of stable and reliable fluid percussing traumatic brain injury(TBI) models, we studied the brain protective effect of Yunnan Baiyao on the model rats by testing Neurological Severity score(NSS) and cerebral edema.Methods:24 SD rats were randomly assigned into three groups:control group, model control group and Yunnan Baiyao(YB) treatment group. Each group contained 8 rats. Model control group and Yunnan Baiyao(YB) treatment group were established moderate or severe TBI model according to fluid percussing method. The YB treatment group was treated with Yunnan Baiyao (100mg/kg,q.d) and warm water, the control group and model control group were only treated with same amount of warm water. Neurological Severity Score (NSS) was evaluated after TBI at 0,3,5day respectively. All the rats were killed at the 5th day, the pathological change and brain water content were tested.Results:1) Fluid percussing TBI model with the pressure 2.0-2.2atm is stable and reliable.2) The NSS had no significant differences between the YB treatment group and model control group at 0d(0> 0.05)。But compared with model control group, the NSS in YB treatment group greatly decreased at 3,5d (P<0.05).3) The pathological change of edema in the YB treatment group was better than the model control group. Compared with model control group, the brain water content in YB treatment group greatly decreased (P<0.05). Conclusion:1) Fluid percussing TBI model with the pressure 2.0-2.2atm is suitable for this study.2) Yunnan Baiyao can reduce the NSS and brain water content of the TBI model rats, which may have therapeutic effect on TBI.Part Two:The effects of Yunnan Baiyao on Caspase-3 expression and neurons apoptosis in Rats after Traumatic Brain Injury.Objective:To explore effects of Yunnan Baiyao on Caspase-3 expression and neurons apoptosis in Rats after TBI.Methods:42 SD rats were randomly assigned into seven groups:control group, 24h,72h,120h model control group and 24h,72h,120h Yunnan Baiyao(YB) treatment group. Each group contained 6 rats. Model control group and Yunnan Baiyao(YB) treatment group were established moderate or severe TBI model according to fluid percussing method. The YB treatment group was treated with Yunnan Baiyao (100mg/kg,q.d) and warm water, the control group and model control group were treated with same amount of warm water. Brains of rats were collected at different time (24h,72h,120h) after perfusion, then we determined the change of Caspase-3 expression and neurons apoptosis in tissues samples of frontal cortex and district in cornu-ammonis by immunohistochemistry.Results:1)The expression of Caspase-3 in YB treatment group was significant lower than that in model control group (P<0.05), and was significant higher than that in control group (P<0.05) at 24h,72h,120h.2)The apoptosis index(AI) in YB treatment group was significant lower than that in model control group (P<0.05), and was significant higher than that in control group (P<0.05) at 24h,72h,120h.Conclusion:There are neuronal apoptosis and change of Caspase-3 expression after moderate or severe TBI. Yunnan Baiyao can significantly restrain the neuronal apoptosis and the expression of Caspase-3 gene, which might have therapeutic effects for treating brain injury rats.
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