Antifungal Effects Of Mongolian Medicine 'Kill-Nian' Recipe

In recent decades, the rising fungal infections in patients with nosocomial infectionshas been the most important cause of death.Fluconazole, amphotericin B are the most important clinical drug of choices for thetreatment of fungal infections ,but prolonged use of these drugs will bring trouble,, such asside effects. it is of great significance to explore new drugs with broad-spectrum anti-fungaldrugs and low toxicity in medical and infection prevention applications.Mongolian Medicine is the national treasure for histories, its treatment of fungalinfections has an unique approach. Mongolian delimit that "stick " is a class of Mongolianmedical causes, judging by the modern scientific point of view, most scholars believe thatincludes the eukaryotic cell, prokaryotic cells and non-pathogenic microorganisms,including microbial cells, including relatively small size of individual Human parasites.The combination of Mongolian medicine theory, kill the "stick" to study the role ofanti-fungal prescriptions, mainly for the following aspects of work:â‘ Mongolian agar holemethod antifungal activity of compound screening;â‘¡by microdilution method singleflavor Mongolian determination of minimum inhibitory concentration MIC;â‘¢systemicinfection in mouse model of Candida albicans;â‘£survival in mice Time, the number ofcolonies and other organs indicators, evaluation kill "stick " prescription drug effect ofanti-Candida albicans.1 Mongolian kill "stick "to the prescription drugs in vitro susceptibility of fungi1.1 Evaluation antifungal activity of Mongolian Medicine using agar hole methodThe inhibition zone size to measure and determine the strain sensitivity of the testeddrugs. The results showed 7-Smilax glabra extract on Candida albicans soup all haveantibacterial activity, while the 7- powers of the pill, sore dry bulk 7 little effect.1.2 Evaluation antifungal activity of Mongolian Medicine using microdilution method.On the basis of the screening,the antifungal activity of 21 kinds of drugs to 10categories of fungal strains were evaluated using the microdilution method to determinetheir MIC values. The results: soil Fuling, abalone, Aconitum show antifungal activityagainst Candida albicans,Candida krusei; myrobalan show antifungal activity againstCandida albicans ; multi-leaf on Oxytropis show antifungal activity against Microsporumcaninum and Microsporum gypseum; vermilion, realgar, Calcitum, cinnabar, borax show broad-spectrum antifungal activity; Toosendan, berberine, Dianthus superbus, showantifungal activity against Candida albicans, Cryptococcus neoformans; compound recipesoup Smilax 7 show the best antifungal activity . This is consistent with the results ofscreening.2 Mongolian killing "stick " prescription drug in vivo.2.1 Establish the systemic Candida albicans infection aminal model of miceTo build a Candida albicans systemic infected model of mice which areimmunosuppressed. The spore suspension of the international standard bacterial strain xwas injected into the mice via lateral tail vein. Previously, we applied hydrocortisone to themice via abdominal cavity to inhibit immune system.2.2 Evaluation of effects of anti-Candida albicans through the survival time of mice,distribution of organ colonyAnimal group to take 6-8 weeks of age, male and female mice 90, 15 in each group,divided into four treatment groups, two control group. 9 days after treatment, the survivaltime in mice. on the survival of infected mice is record,Candida albicans can be seperatedfrom the kidneys of the mice using sabouraud medium,,results show: the highconcentration of drug group show significantly different(p <0.05) on the survival timecompared with the control group, facilitate that recipe soup Smilax 7 may prolong thesurvival time of Candida albicans systemic infected, but compared with ketoconazoletreatment group also showed significant differences (p <0.05), that the antifungal effect ofrecipe soup Smilax 7 has not yet reached the level of ketoconazole.In summary, by in vivo and in vitro drug sensitivity test we show that Mongolianrecipe soup Smilax 7 show great antifungal activity against Candida albicans; and 18 typesof single and three kinds of taste of Mongolian medicine prescriptions to 10 Kinds ofclinical common systemic infection, are Investigat soil Fuling, abalone, Aconitum showantifungal activity against Candida albicans, Candida krusei ; myrobalan show antifungalactivity against Candida albicans ; multi-leaf on Oxytropis show antifungal activity againstMicrosporum caninum and Microsporum gypseum; vermilion, realgar, Calcitum, cinnabar,borax show broad-spectrum antifungal activity; Toosendan, berberine, Dianthus superbus,show antifungal activity against Candida albicans, Cryptococcus neoformans; This isconsistent with the results of screening. Through the establishment of systemic infection inmice model of Candida albicans on Qiwei soup Smilax evaluate in vivo antibacterial,indicating that may prolong the survival time of Candida albicans systemic infected. This study may also facilitate the deeper understanding of Mongolian kill "stick " for the usein the antifungal prevention applications...