Effects Of NXKⅡHKFY On SOD, GSH-PX, MDA Myocardial Ischemia In Rats

Myocardial ischemia is all causes coronary artery blood flow is reduced, causing myocardial oxygen substances supplies and metabolites cleared to reduce or accumulation. Long time of myocardial ischemia can cause myocardial infarction is one of the most serious myocardial ischemic diseases. Current thinking is the main mechanism of myocardial ischemia injury oxygen free radical damage, including intracellular calcium overload, energy metabolic disorders, endoplasmic reticulum stress, inflammation, cell apoptosis. Myocardial ischemia drug therapy is key to make oxygen supply and consumption to regain balance, its traditional treatments mainly divided into two categories. One is to reduce myocardial oxygen consumption; Another kind is to improve coronary blood flow. Modern medical treatment of this disease using fibrinolytic therapy, percutaneous coronary angioplasty, coronary artery expansion in art, coronary artery bypass procedure to wait, all have achieved good therapeutic effect. But for myocardial ischemia, traditional Chinese medicine has early prevention first "is not ill lead defense, has been sick features, plenty of clinical data and the practice proves that many more single traditional Chinese medicine not only can delay the onset of coronary heart disease, the more important is the process of early prophylaxis can delay the onset ages, even reduce the incidence of the disease. NXKⅡHKFY isⅠbranch newly developed of coronary heart disease prevention and control, the experiment by establishing prescriptions and similar animal myocardial ischemia model, to observe NXKⅡHKFY of myocardial ischemia effect, and discuss the relevant mechanism for the drug clinical application, provide experimental basis.This experiment will randomly divided into six groups of rats, i.e. normal controls, negative control, positive control, NXKⅡHKFY large, medium, small doses groups, each group of 10 only. To rat stomach dosing filling after 20 days, the PIT 2 consecutive times injections of rats, celiac copy myocardial ischemia model, in second injection PIT30 minutes after myocardial ischemia, determine the electrocardiogram monitoring after a successful model replicates, abdominal aortic anesthetized rats, take some blood, centrifugal take supernatant fluid, test serum SOD, GSH-PX vitality and the MDA content, HE dyed observation myocardial tissue pathology.The results show:1, negative control, electrocardiogram myocardial ischemia in rats 90%, with normal performance group compared (P＜0.01), build mode success. And NXKⅡHKFY each dose group were significantly lower rate of myocardial ischemia in positive reaction, and the degree of myocardial ischemia in control group for light is also negative, indicating that NXKⅡHKFY each dose group of all can effectively improve the abnormal electrocardiogram. One NXKⅡHKFY high-dose group is the most significant difference (P<0.01) and positive rcpmaj no significant difference (P>0.05). 2, SOD change:the normal control group compared with control group, the negative that dropped significantly, serum activity in myocardial ischemia injury of SOD plays an important role; NXKⅡHKFY large, medium and small dose group and negative rcpmaj, were significantly increased; SOD activity.NXKⅡHKFY three groups, a large dose of elevated SOD activity of low-dose ability is strong, and large doses of small doses of SOD activity group compared with significant difference (P<0.05). Medium and small dose group of SOD were lower than positive group; Big dose group of SOD and positive rcpmaj, no significant difference (P> 0.05). NXKⅡHKFY above show that can be significantly elevated serum myocardial ischemia in rats in the activity of SOD, namely can strengthen endogenous oxygen free radical scavenging system function, thus reduce the damage of myocardial oxygen free radicals. 3.MDA content changes:compared with normal control group, negative controls significantly higher serum MDA content; NXKⅡHKFY large, medium and small dose group and negative rcpmaj, were significantly lower serum MDA content; Dose group of big, in small doses groups, MDA below are significant difference (P< 0.05), and big dose group of MDA content below, the differences in dose group of significant (P< 0.05). Medium and small dose group and positive rcpmaj, MDA content are significant difference (P<0.05), medium and small dose group of MDA content were higher than positive control; High-dose group and positive rcpmaj, MDA content without significant difference (P> 0.05). The results show that NXKⅡHKFY can significantly reduce serum myocardial ischemia in rats, MDA content that could reduce lipid peroxidation products so as to reduce the MDA content of myocardial ischemia injury.4, GSH-PX vigor changes:normal control group compared with control group, negative serum GSH-PX vigor declined obviously, explain GSH-PX myocardial ischemia injury in plays an important role; NXKⅡHKFY large, medium and small dose group and negative rcpmaj, were significantly increased GSH-PX vigor; NXKⅡHKFY three groups, medium and small dose group of group compared with large doses, GSH-PX vigor are significant difference (P<0.05), medium and small dose group of GSH-PX vitality are below high-dose group, in small doses group and dose group of comparison, GSH-PX vigor non-significance difference (P> 0.05). NXKⅡHKFY above show that can obviously increase myocardial ischemia in rats serum GSH-PX vigor, namely can enhance the function of free radical scavenging system so as to reduce the damage of myocardial oxygen free radicals.5, And pathological change:myocardial ischemia in rats ischemic heart tissue morphologic changes in visible myocardial fiber fracture, muscle swelling of the horizontal grain disappear, organization clearance, widened obviously widespread, inflammatory cell nuclear become pyknotic. NXKⅡHKFY large, medium and small dose group and positive and negative control group compared, all can reduce myocardial tissue damage, myocardial arrangement is neat, swelling degeneration is lighter, less inflammatory cell. Proof NXKⅡHKFY has reduces the role of myocardial cell damage.To sum up:NXKⅡHKFY can effectively improve the abnormalECG, stable myocardial cell electrophysiology. NXKⅡHKFY through elevated serum myocardial ischemia in rats of SOD, GSH-PX activity and reduce the MDA content and strengthen the oxygen free radical scavenging system function, improve the body anti-oxydation ability, reduce oxygen free radical production, restrain lipid peroxidation product formation, thus reduce the damage of myocardial oxygen free radicals, to reduce the role of myocardial ischemia injury.