Expressionand Clinical Significance Of OPN And VEGF In Keloid

Objective:To observe the expression of osteopontin (OPN) and vascular endothelial cell growth factor (VEGF) in keliod, normal scar and normal skin. And to explore their influences on the development of keliod.Methods:The samples were collected from 59 patients, in which there were 20 with keliods,23 with normal scars and 16 with normal skin tissues as control. After paraffin imbedding, cutting and staining, immunohistochemical method (SP method) combining computer pathological image analysis were carried out to detect the expression of OPN and VEGF in each section. The positive rate and integral optical density (IOD) of OPN and VEGF were analysed to study the differencies and dependability in three groups. All statistical analyses were carried out by using the statistical analysis software package SPSS 13.0.Results:①The positive expression rates of OPN are 95% in the keliod group,52.17% and 0 in the normal scar group and normal skin group respectively. The correlated IODs of the 3 groups are 455103.75±145907.22,243162.05±54810.94 and 38952.65±8216.19. The expression of OPN in keliods was remarkably significant in comparison with normal scars and normal skin tissues (P<0.01).②The positive expression rates of VEGF are 80% in the keliod group,17.23% and 0 in the normal scar group and normal skin group respectively. The correlated IODs of the 3 groups are 224321.9±50038.73,17339.02±5776.16 and 5501.54±677.27. The expression of VEGF in keliods was remarkably significant in comparison with normal scars and normal skin tissues (P<0.01).③There was positive correlation existing between OPN and VEGF in keliods (P<0.05).Conclusions:①The over-expression of OPN may be related to the formation of keloid.②Positive correlation exists between OPN and VEGF in keliod.