Experimental Research Of The Treatment Of Mycophenolate Mofetil On IgA Nephropathy

Objective:To establish the IgAN rat model, observe the effect of the model by Mycophenolate Mofetil and explore the improved IgAN mechanism.Method:To choose SPF six weekly forty male Wistar rats, randomly divided into five groups:model group, Captopril group, Mycophenolate Mofetil group, Captopril + Mycophenolate Mofetil group and the normal group. IgA nephropathy model was induced by the improved method of bovine serum albumin (BSA)+lipopolysaccharide(LPS). The Captopril roup, Mycophenolate Mofetil group and Captopril+Mycophenolate Mofetil group were treated with Captopril, Mycophenolate Mofetil and Captopril + Mycophenolate Mofetil from ninth week, once a day, to twelfth weekend. At the end of the experiment, the rats were killed to these observations:First, the rat general state. Second, urine RBC were detected by microscopic examination, urine protein were detected by Coomassie brilliant blue. Third, automatic biochemical analyzer detection of renal function, Liver function, blood fat. Four:Immunohistoche-mical detection of renal MMP-9, TIMP-1 content. Five:optical microscope, fluorescence microscope and electronmicroscope to observe animal model of renal pathology.Results:First:General state:The normal group rats were well in spirit,reactive activities and eating, without obvious abnormalities; The model group and the treatment group rats reduced activities of different levels, eating few, fluffy hair, looseness of bowels and so on. The model group were typical and last the longest time. The treatment group were convalescence about one week and the performance were lighter than the model group.Second:The detection of RBC and proteins in urine:the normal group has not appeared hematuria and proteinuria. The hematuria and proteinuria of treatment groups were reduced compared with the model group. And the difference was statistically significant (P<0.05). The Captopril+Mycophenolate Mofetil group and the Captopril,Mycophenolate Mofetil group was statistically significant (P<0.05). The Captopril group and the Mycophenolate Mofetil group was not significant (P>0.05).Third:the detection of renal function, Liver function, blood fat. Each groups has not enough difference. And the difference was not significant (P>0.05).Four:the nomal group rats in the optical microscopy showed mesangial cells, the capillary loop open. The treatment groups pathological changes were less serious than those of model group. Fluorescence microscopy revealed normal glomerular mesangial area are non-IgA deposition; The treatment groups glomerular and tubular IgA showed granular yellow-green fluorescence; the Captopril+Mycophenolate Mofetil group were the lightest. Electronmicro-scope showed the normal group the foot process normal, the basement membrane integrity. The treatment groups changes were less serious than the model group. The MMP-9 levels in the show is that the treatment groups and model group were significantly higher. Compared with the control group was statistically significant (P<0.05). The Captopril+Mycophenolate Mofetil group and the Captopril, Mycophenolate Mofetil group was statistically significant (P <0.05). The Captopril group and the Mycophenolate Mofetil group was not significant (P>0.05). The TIMP-1 levels in the show is that the treatment groups were significantly lower.Compared with the model group was statistically significant (P<0.05). The Captopril+Mycophenolate Mofetil group and the Captopril, Mycophenolate Mofetil group was statistically significant (P <0.05). The Captopril group and the Mycophenolate Mofetil group was not significant (P>0.05).Conclusion:Mycophenolate Mofetil can improve IgA modified rat model's symptoms, reduce urinary protein and urine RBC, and through accommodate MMP-9, TIMP-1 expression, inhibition extracellular matrix accumulation and reduce glomerular and tubular-interstitial injury. This maybe its mechanism of action. Captopril and Mycophenolate Mofetil these two kind of drug combination to treatment IgAN is better than Captopril or Mycophenolate Mofetil alone.