| Objective To investigate the protective effect of mycophenolate mofetil (MMF)on renal lesions of early stage in experimental diabetic rats and explore its mechanism. Methods Healthy male Wistar rats were randomly divided into three groups after uninephrectomy: C, a control group;DM,rats made diabetic with streptozotocin;DM+MMF,diabetic rats treated with MMF,10mg/kg once daily by gavage .The treatment lasted for 8 weeks.Creatinine clearance and urinary albumin excretion were measured at 2 week, 4 week and 8 week.Renal pathological changes were evaluated and immunohistochemistry was used to examine the infiltration of macrophage and lymophocyte in the kidney at 8 week. Result Compared with DM group, urinary albumin excretion in DM+MMF group was markedly decreased at 8week(DM:146.2833±64.2944μg/24h vs DM+MMF:53.59±16.3928 μ g/24h,p ﹤ 0.05),so was Creatinine clearance(DM: 7.3270±2.9445 ml.min-1.kg-1 vs DM+MMF: 4.0263±0.8784 ml.min-1.kg-1 ,p﹤0.05). Pathohistological study revealed that MMF inhibited the diabetic-induced expansion of mesangial area and thickening of GBM. Immunohistochemistry showed that the kidney of diabetic rats exhibited marked macrophage infiltration at 8 week and MMF treatment could prevent macrophage infiltration(DM:7.29±2.14cells/gcsvs DM+MMF:4.07±2.05cells/ gcs, p﹤0.05). The rats of three groups were not examined lymophocyt infiltration. Renal lesion was correlated with glomerular macrophage infiltration (r=0.685,p﹤0.05). Conclusion MMF may prevent the progression of diabetic nephropathy through its anti-proliferative and anti-inflammatory properties.
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