Oct-4 Expression In Epidermal Tumors

Objective:The aim of this study was to understand the role of the expression of specific markers of stem cells Oct-4 in the development of human epidermal non-melanoma cutaneous tumors.Methods:The paraffin-embedded samples were retrieved from files of pathology department at our hospital, including 20 cases of skin squamous cell carcinomas(SCC),20 cases of basal cell carcinomas(BCC),20 cases of seborrhoeic keratosis(SK) and 20 cases of normal skin(from head,face,trunk,extremities and the like). The expression of Oct-4 and PCNA were observed by immunohistochemical staining technique.Results:â‘ Oct-4 protein was abnormally increased in SCC and BCC as compared to normal skin and SK (p<0.05). However there were no significant difference of Oct-4 protein expression between SCC and BCC (p>0.05). There were also no significant difference of Oct-4 protein expression between Sk and the normal skin(p>0.05),and no significant difference of Oct-4 protein expression between SK and BCC (p>0.05)â‘¡PCNA protein was abnormally increased in SCC and BCC as compared to normal skin and SK (p<0.05). There were significant difference of PCNA protein expression between SCC and BCC(p<0.05). There were also significant difference of PCNA protein expression between SK and the normal skin(p<0.05). However there were no significant difference of PCNA protein expression between SK and BCC (p>0.05).â‘¢There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC.Conclusion:â‘ The abnormal expression of Oct-4 may have an important role in the development of BCC and SCC. Positive Oct-4 expression cells may be the tumor stem cell in SCC and BCC.â‘¡There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC. The over expression Oct-4 in BCC and SCC may play an important role in proliperation of tumor.