Protective Effect Of Lisinopril On Rat EAE And Ngb, C-jun Protein Expression

Objective To establish the animal model of experimental autoimmune encephal- omyelitis(EAE) and observe the expression of neuroglobin and C-JUN in spinal cord of EAE rats, explore the possible protective function mechanism of lisinopril(L) to EAE rats, seek for the efficient cost-effective way of multiple sclerosis's treatment and new theoretical basis.Methods 90 female SD rats were randomly divided into five groups: normal group (CON group), complete Freund's adjuvant (CFA group), EAE model group (EAE group), L1 group (EAE + L2mg/kg.d), L2 group (EAE + L20mg/kg.d), each group was divided into three subgroups; Then, the neurological function of rats was observed and pathology, neuroglobin (Ngb) and C-JUN protein expression was observed by brain tissue sections.Results1.Incidence of a disease: The rats of CON and CFA groups were without disease; Incidence of EAE group was 88.89%; Incidence of L1 and L2 groups were significantly lower than the EAE group (P <0.05). the incidence of L2 group is lower than the L1 group (P <0.05).2.Ethology appraisal: Neurological function scores of the L1 and L2 groups were lower than the EAE group (P <0.05). Neurological function scores of L2 group were less than the L1 group (P <0.05).3.Histopathological findings: The brain of rats of the CON and CFA groups were without inflammatory cell.The number of vascular cuff of the L1 and L2 groups were less than the same period of the EAE group (P <0.05);The L2 group was less than the same period of the L1group (P <0.05). 4.Neuroglobin and C-JUN expression: Neuroglobin and C-JUN cells could be seen in each group. Expression of the C-JUN and Ngb of the CON and CFA groups were lower than the EAE group, L1 group and L2 group (P <0.05); And the L1 and L2 groups were lower than the EAE group (P <0.05). The L2 group is lower than the L1 group (P <0.05).Conclusion1.The inflammatory lesions was few relatively,where the parts included more Ngb in the model of EAE. It explained Ngb may have the protective effect on EAE.2. Expression of C-JUN protein is changed with the degree of EAE.3. Lisinopril could reduce the incidence of EAE and inhibit the expression of protein C-JUN.When condition of the EAE improved,expression of Ngb reduced naturely. The lisinopril may have a protective effect on EAE. and the protective effect may be stronger with the greater dose.