| Objective:To study the protective role of adiponectin against the doxorubicin-induced inhibition in the proliferation of H9C2 myocardial cells and investigate the protective mechanism of adiponectin in anthracycline antineoplastic drug induced myocardial oxidative stress injury.Methods:The protective effect of adiponectin against doxorubicin induced inhibition of rat myocardial cells H9C2 proliferation was measured by MTT method. H9C2 cells were cultured in vitro, treated by adiponectin with a series of concentrations (1μg/ml,5μg/ml,10μg/ml) for 18 hours and 1μmol/L doxorubicin for 6 hours. The control group,adiponectin group and doxorubicin group were detected by the absorbance of 630nm by MTT assay for the cell relative viability.Results:MTT assay showed that adiponectin has no significant effect on the relative viability of rat myocardial cells H9C2 (P>0.05); while doxorubicin had an anti-proliferative effect on H9C2 (P<0.05); but adiponectin was found to strongly degrade the anti-proliferation effect of doxorubicin on H9C2 in a dose-dependent manner (P<0.05)Conclusion:Adiponectin shows significant inhibitory effect on the anti-proliferation effect induced by doxorubicin on rat myocardial cells H9C2 in a dose-dependent manner. Adiponectin probably has protective effects on anthracycline-induced myocardial toxicity. |
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