| Objectives To explore the association of transforming growth factorβ1(TGF ) gene +869 polymorphism and serum concentration of TGFβ1 with congenital heart disease (CHD) in children and analysis the relationship of myocardial fibrosis between genotype, allele frequency and serum concentration of TGFβ1 with CHD.Methods We collected 41 patients who were proved to be CHD by supersonic inspection aged form 3 months to 9 years old and matched with 33 healthy children aged form 5 months to 7 years old as control group. Extracted blood was separated to single nuclear cells and plasma, and used separately to analyze the polymorphism of TGFβ1 gene +869T/C by polymerase chain reaction restricted fragment length polymorphisms method (PCR-RFLP) and determine by enzyme-linked immunosorbent assay (ELISA). SPSS 16.0 software was used for the statistical analysis of these data.Results①Gene type distributions in congenital heart disease and control group abide by Harding-Weinberg balance;②Serum concentration of TGFβ1 in CHD is significantly difference than the control group (P<0.01);③There was no statistically difference between different kinds (VSD, PDA, ASD) of CHD(P>0.05);④Compared with control group, gene type and allele frequency distribution has a statistical significance difference in CHD(P＜0.01);⑤Serum concentration of different genotypes of TGFβ1 have a more statistical significance in CHD than control group, presenting the trend of TT |
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