The Study About Correlationship Between Congenital Heart Disease And Mthfr Gene Polymorphism And Environmental Factors

Objectives:(1) To investigate the correlationship between 5,10-methylenetetra hydrofolate reductase(MTHFR)'s C677T genetic polymorphism and environmental factors. (2) To study the correlationship between parents'mutations of MTHFR C677T gene and their children's. (3) To assess the environment risk factors of CHDand to discuss the methods to prevent CHD.Methods:(1)A paired case-control study (case=100 control=200) was used. Cases and controls were paired according to age, sex and living area. Cases were correctly diagnosed by clinical and colored ultrasonic.To study MTHFR C677T genotype distributing and difference between children and his mother by polymerase chain reaction restricted fragment length polymorphism (PCR-RFLP) and enzymatic cleavage.To analyze the chance of mother transmit two alleles of gene C677T of MTHFR to their children. To detective if the change of MTHFR gene C677T C to T is higher than control group. (2)Design The investigate questionnaire of new born baby, Investigate the parents of cases and controls by visitation and interview to obtain the material whether they have contacted the relevant risk factors or not before and during pregnant period。(3)Use simple Conditional Logistic regression analysis to analyze 28 possible risk factors for CHD,and multi-conditional Logistic regression analysis was used to analyze the 16 selected risk factors associated with CHD.In the same time, we use factor analysis, according the proportion of the factor contribution to find the common factors.Results:(1) In case group, The change of C to T of MTHFR gene's C677T gene is higher than control group; (2)Mother's C677T gene has some relation with children; (3) We can find risk factors from multi-conditional Logistic regression analysis, such as:Pregnancy-induced hypertension syndrome, Antipyretics, Pesticide exposure condition, Early pregnancy meal quality,the amount of vegetable intake,Negative life events,the amount of alcohol taking, the genotype of children. The odds ratio are:29.76,10.194,15.156,0.414,4.69,6.302,2.333,3.474。(4) We can find common factors by factor analysis, they are:Dietary factors, Negative life events factor,i Cold-related factor, smoking and alcohol drinking factors, Baotai relevant factor, pesticide and vegatable factors, pregnancy complications factors, genetic factors.Conclusion:(1)The mother's MTHFR Gene has some relation to their children. (2)The change of MTHFR C677T Gene is correlated to CHD. (3)There are some correlations between Environmental factors (such as Dietary, infection, negative life events, smoking and alcohol drinking, pesticide exposure, and pregnancy complications) and CHD. It is possible to preventing CHD by improving environmental factors.