Expression And Significance Of Microtubule-associated Protein 1 Light Chain 3(lc3a And Lc3b) In Breast Carcinoma

Purpose To investigate the expression levels of isoforms of Microtubule-associated Protein 1 Light Chain 3 ( LC3A and LC3B) and their mRNA in breast carcinoma,and to explore the relationship between LC3A and LC3B and the pathogenesis and metastasis of breast carcinoma.Methods SP immunohistochemical and RT-PCR method were used to detect the expression levels of LC3A,LC3B and their mRNA in the normal breast tissues in 8 cases,breast proliferative diseases(epithelial hyperplasia)in 20 cases,breast preinvasive carcinoma in 10 cases and breast invasive carcinoma in 40 cases.Results (1)There was significant relationship between LC3A and LC3B staining (intensity and proportion) and type of tissues.(2)Both LC3A and LC3B mRNA were showed significant increasing gradient of expression levels hyperplasia ), breast preinvasive carcinoma and breast invasive carcinoma. Based on WHO grade, LC3A mRNA levels were showed significant increasing gradually in cancer specimens withâ… ,â…¡andâ…¢grade(P<0.05). (3)There was no significant relationship between LC3 expression and clinical stage, age group and lymph node metastasis.Conclusion LC3A protein and expression occurred in greater abundance and in a larger percentage of cells in breast cancer tissues as compared with the normal tissue, and LC3A mRNA levels were showed increase gradually in cancer specimens withâ… ,â…¡andâ…¢grade, suggesting that LC3A isform maybe related to the occurrence and development of human breast cancer.