Experimental Study On Yinhuang Solution On Bladder Mucosal Injury In Rat

There is a surface protection material on normal bladder mucosal (GAG layer) [1], it can prevent the inflammatory substances, clumps of bacteria, carcinogenic substances adhesion to the surface of bladder mucosa. When this protective mechanism was destroyed for various reasons, bladder mucosa will be damaged , causing acute inflammation, Bladder damage caused by acute bladder inflammation, currently there is no good control methods at home and abroad. As qingrejiedu chinese traditional medicines honeysuckle and skullcap, skullcap with a heat dampness, purging fire detoxification, hemostasis, anti-bacterial, anti-inflammatory and other effects. Honeysuckle inhibit a variety of bacteria including E. coli , and promote the dissipation of inflammatory cells, as well as antioxidant effects. Compound composed of two yinhuang solution with a significant antibacterial, antiviral, antipyretic, anti-inflammatory and immune regulation, has significant clinical efficacy [2].Before and /or after acute injury of hydrochloric acid solution to the bladder mucosa of rats, perfusion with yinhuang solution .Observe the morphology of bladder mucosa, inflammatory cell infiltration, the bladder mucosa of heat shock protein 70 (HSP70) expression. To investigate the protection effects of yinhuang solution on he bladder mucosa of rats. To explore its efficacy and mechanism of action.ObjectiveTo investigate the effects of yinhuang solution on the bladder mucosal before and/or after mucosal injury. Methods32 SPF female SD rats were randomly divided into 4 groups with 8 rats in each group. Group A was used for the normal control group, group B for the model control group, group C for preventive group, group D for preventive and therapeutic group. Injured rats were created by hydrochloric acid applied on bladder mucosa. After the models were established or before and after the models were established, bladders were perused with yinhuang solution. Severity of inflammatory infiltration were observed by HE staining, histological score and heat shock protein70 (HSP70) expressed in the mucosa.ResultsEndoscopic pathological changed, group B had a large number of inflammatory cell infiltration, significant expansion of capillary, epithelial cell necrosis. Group C had a small amount of expansion of capillary, inflammatory cell infiltration. Group D scattered in expansion of capillary, a small amount of inflammatory cell infiltration. Concerning the results of histological score and HSP70 expression, there was a significant difference between group A and groups B, C and D (P<0.01); there was a significant difference between group B and groups C and D (P<0.01); there was a significant difference between group C and group D (P<0.05).Conclusion:Yinhuang solution can evidently improve the repair capability of bladder mucosa after injury.