Vegf, Id-1 And Microvessel Density With Spinal Metastases Of The Relationship Between Blood Loss

Objective: Surgical treatment of metastatic spinal tumor treatment is a vital part in the surgical procedure, blood loss is too large the doctor frequently encountered problems. Spinal vertebrae from various sources by detecting tumor VEGF, Id-1, and the expression of angiogenesis to investigate the blood loss and patients with clinical prognosis, prediction of clinical preoperative blood loss, preoperative preparation guidance, prognosis, provided new theoretical basis.Methods: Immunohistochemical staining to detect Id-1, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in 32 cases of spinal metastasis tissues, using statistical software SPSS 13.0 comparing different sources of blood loss during spinal metastases the amount of its indexes and clinical sources of blood loss and the relationship between the tumor.Results:1 Thirty-two cases of spinal tumors from different sources had different levels of Id-1 expression, the positive rate was 100%. Through statistical analysis, Id-1 had significant correlation between tumor sources, P = 0.022 (<0.05). The expression of Id-1 correlated with the clinical blood loss, which had a statistically significant difference (P <0.05), and after a spearman correlation r_s = 0.230, P <0.05, a positive correlation, suggesting that Id-1 with the expression increased, blood loss tended to increase. The expression of Id-1 with gender, age, had no significant difference between incidence of vertebral body.2 VEGF as a target for angiogenesis in various tumor tissues were expressed to varying degrees. Obtained through analysis, VEGF and tumor origin was statistically significant, p = 0.013 (<0.05). VEGF and clinical statistical analysis of blood loss, p = 0.016 (<0.05), statistically significant. After spearman correlation r_s = 0.322, P <0.05, a positive correlation, suggesting angiogenesis as the cells increase the amount of clinical blood loss also increases. With gender, age and incidence of vertebral comparison, statistical analysis revealed no statistical significance.3 Microvessel density, The expression of CD34 was selected as the observation of the classic indicators, different sources of spinal metastasis, the immunohistochemical staining method using Weidner count, results and pathological features of spinal metastases were compared. In epithelial and mesenchymal origin in the spinal metastasis, CD34 MVD results were shown (30.2±12.5) and Article (40.6±11.2) section, range was 20 to 120 and 15 to 160, the difference statistically significant (P <0. 0.05).4 Rank sum test analysis of Movement and mesenchymal-epithelial source of blood loss of spinal metastases, p = 0.035 (<0.05), the difference was statistically significant, large bleeding tumors of epithelial origin.Conclusion:1 Comparison of spinal metastatic tumor hemorrhage, epithelial mesenchymal origin was significantly higher than the source. Clinical preoperative preparation can be made accordingly appropriate preoperative preparation.2 Id-1 expression and spinal metastases from relevant. Id-1 expression of the corresponding group also tended to increase the amount of bleeding, a positive correlation between.3 VEGF showed high expression of angiogenic activity, the source of its correlation with tumor volume and blood loss correlated, suggesting that blood vessel formation with increased blood loss with the increasing trend.4 Different sources of spinal metastasis, microvessel density is different from the transfer of tumor vascular epithelial richer.