The Expression And Significance Of Estrogen Receptor Subtypes And Progesterone Receptor In The Tissue Of Hyperthyroidism

Objective:Hyperthyroidism is a common endocrine diseases in clinical, the main change is abnormal increasing secretion of Thyroid Hormone.The main symptom is hypractive metabolism in whole body. The most common one is primary hyperthyroidism, but the etiology is not completely understood. Genetic, environmental and immune are considered as the reason of hyperthyroidism until now, but the opinions have not unified. In clinical found that most patients of hyperthyroidism are middle-aged women with exuberant endocrine, whether this exuberant in women have a relationship with hyperthyroidism worth exploring.In recent years the effect of Estrogen and progesterone in stimulating tumor is preliminarily determined, but whether express in hyperthyroidism and whether play a part in the disease remains unclear. And with the further research of estrogen receptor subtypes (ER alpha, beta), people found ERαhave very different from ERβin distribution, function and regulation, whether the endocrine therapy for breast cancer with sex hormone receptors positive can be used for hyperthyroidism worth discussing. Studying the expression of estrogen receptor subtypes on hyperthyroidism can be help exploring the pathogenesis of hyperthyroidism. These studies detect the expression of estrogen receptor subtypes (ER alpha, ER beta) and progesterone receptor (Pr) on hyperthyroidism organization by immunohistochemical method on the basis of research achievements around the world. Analysis whether the expression of estrogen receptor subtypes (ER alpha, ER beta) and progesterone receptor (Pr) have some relation with clinical and pathological features of hyperthyroidism. Exploring the mechanism and function of estrogen receptor subtypes (ER alpha, ER beta) and Estrogen receptor (Pr) in the hyperthyroidism, providing theoretical basis for the endocrine therapy of hyperthyroidism. Methods:1 case data: This experiment take 104 cases paraffin sliced of hyperthyroidisms from department of glandular surgical in The second hospital of hebei medical university between October 2008 and October 2010 for the test group, take peritumoral tissue of another 60 patients with Thyroidadenomas as control group. preoperative Diagnosis of all patients is primary Hyperthyroidism, postoperative pathologic diagnosis are toxic goiter, include 16 cases of men and 88 cases of women, with the average ages of 29 years old, medianage 26.5 year.2 Experimental methods: taking immunohistochemiatry streptavidin- peroxidase (SP) method to detect the expression of ER alpha, ER beta, Pr in 104 of cases hyperthyroidism and 60 cases of Thyroidadenomas. Analysis the association of expression of ER alpha, ER beta, Pr and hyperthyroidism clinical pathologic characteristics, taking correlation analysis of ER alpha, ER beta, Pr.3 Statistical analysis: statistical processing using the SPSS13.0 statistical software package, using X2 inspection analysis. Taking a=0.05 for the statistical inspection standards. Taking Pearson's coefficient of meansquare contingency for correlation analysis. C=0 means fully independent, C=1 means completely related.Results:ER alpha, ER beta, PR mainly distributed in the nucleus, for tan or auburn grain, cytoplasm also contains a few receptors, most positive cells for thyroid follicular endothelial, stromal cells near the follicular cells is much for negative cells.1 The positive rate of ER alpha, 23.1% for hyperthyroidism (24/104), and 26.7% (16/60) for normal tissue, There were no statistical differences between the hyperthyroidism group and the normal group (P > 0.05) . Detect expression of ER alpha By gender, age group in the 104 cases hyperthyroidism organization, and the results show that There are no statistical differences between the two groups (P > 0.05). 2 Detect positive rate of ER beta, in 104 cases hyperthyroidism organization , positive rate is 73.1% (76/104), 46.7% (28/60) positive in normal tissue, there are statistical differences between the two groups (P < 0.05), positive rate of ER beta in hyperthyroidism group is higher than that of normal tissue; positive test in the gender group showed that 16 cases of male hyperthyroidism patients with positive rate of 50% (8/16), women is 77.3% (68/88), there were statistical differences between the two groups (P < 0.05).3 The positive detect of PR, in 104 cases of patients with hyperthyroidism, is only 4 cases, 100 cases negative, the positive rate is 3.8% (4/104). Positive rate in 60 cases of patients with normal tissue is only 5.0% (3/60), there are statistical differences between the two groups (P > 0.05); there are no statistical differences in testing by gender or age group (P > 0.05).4 ER beta has no significant correlation with ER alpha. Conclusion:1 The experiment detect ER beta express higher in hyperthyroidism, positive rate of ER alpha and Pr is low.It's suggestted that hyperthyroidism mainly express ER beta, though ER alpha and Pr express, but for the influence of experiment condition, whether hyperthyroidism express ER alpha and Pr needs to be explored2 Expression of ER beta in hyperthyroidism organization is higher than normal thyroid organization, female higher than male patients, and positive in endocrine exuberant young woman higher than other age groups. Estrogen receptor's increasing expression may be one of the reasons of hyperthyroidism, and may be related to Age and gender.3 The regulation for expression for ER alpha, ER beta, Pr ER maybe is independent. Regulatory mechanism for ER beta still needs further research.