Pharmaceutical Research Of Fufangchuanbeizhike Granules

Fritillaria wabuensis is the new source of drugs F.sungbei Hsiao et K.C.Hsia. from"Chinese Pharmacopoeia"(First Edition 2010),which is the main products of Sichuan earthquake reovery reconstruction.Under the guidance of Chinese medecine,to adopt the main clinical experience prescription with Fritillaria wabuensis side supplide by the famous Professor Lei Zaiquan. According to the physical and chemical properties of prescription drugs,pharmacological efficacy studies,clinical medication repuirements,the prescription formulation screening to determine the compound Chuanbei prescription drugs,pharmacological efficacy studies,clinical medication requirements, the prescription formulation screening to determine Fufangchuanbeizhike Granules, and thus the preparation process, quality standards,the initial stability study and the basis for the Fritillaria wabuensis to the chemical composition of the initial system comparison study.To do the comprehensive comparision of chemical composition of the following studies between Fritillaria wabuensis and the best clinical cough medicine—F.sungbei:①To analyze the overall chemical composition by TLC;②With acid dyes-UV-visible spectrophotometry of Total Alkaloids in both contents and comparative analysis;③To determine the contents of peimine and peininine from different Fritillaria,to study comparative analysis by HPLC-ELSD;④To study the fingerprint of Fritillaria wabuensis and Fritillaria sungbei by HPLC-ELSD;⑤To determine the polysaccharide content of front and back acid hydrolysis of different Fritillaria by phenol-sulfuric acid method,as a baiss,to start the prelimianry analysis of different non-Fritillaria Alkaloids.Fufangchuanbeizhike Granules is processed by Fritillaria wabuensis,according to the physical and chemical properties of effective composition,the use of modern processing technology and clinical application needs,the more comprehensive survey of selected indicators,and ultimately Chuanbei cough compound identified parameters of particles. After the trial production verification,the process is reasonable, stable and feasible.Quality standards of research mainly consists of raw materials and preparation. In accordance with the "Chinese Pharmacopoeia" (2010 edition)pieces of the feed ingredients mainly be inspected in the quality standards of raw materials.Quality standards in preparation for pilot production of three batches of samples were carried out according to the pharmacopoeia of the general particle inspection, qualitative identification,the contents of baicalin and hesperidin were measured simultaneously to control the quality of the sample.TLC method of Fritillaria wabuensis in compound was established with peimine as reference;TLC methods for Aster and Stemona in compound with the corresponding reference drug were established; simultaneous determination of baicalin and hesperidin to multi-target control.Determination of the three batches in the test samples were compared,the transfer rate was more than 50%,indicating less loss of the production process.Three batches of samples were left to be observed in the room temperature,the results showed good stability of Fufangchuanbeizhike Granules. The results of Fufangchuanbeizhike Granules can expand clinical resources,to provide market for cultivating Fritillaria wabuensis to reconstruct the disaster areas in Sichuan,to help people rebuilding their homes.