Determination Of Ester Alkaloids In Radix Aconite Lateralis And Study On The Dynamic Variation Patterns In The Decoction Process

Radix Aconiti Lateralis Praeparata is the side root process production which derive from perennial herb Ranunculaceae plant Aconitum carmichaeli Debx. It was first recorded in《Shennong's Classic of Materia Medica》. Radix Aconiti Lateralis Praeparata is the representative of toxic herbs, acrid and sweet in taste, extremely hot in nature. It acts on the heart, spleen and kidney channels with the efficacies of restoring yang and rescuing patient, tonifying fire and helping yang, dispeling cold and relieving pain. Many doctors in generations consider that Radix Aconiti Lateralis Praeparata is one of the most important traditional Chinese medicines which have the recuperation vitality efficacy. The ester alkaloids are the main toxic and effective components of Radix Aconiti Lateralis Praeparata, including mesaconitine, aconitin, hypaconitine, benzoylmesaconine, benzoylaconine, benzoylhypaconine and so on. People can reduce its toxicity by processing, decocting or combining with other mecidine. But the differences of ester alkaloids contents are due to the differences of processing technologies and sources. Therefore, it's necessary to determine the contents of diester-type alkaloids, mono-ester type alkaloids and total ester-alkaloids for ensuring its safety and efficacy in use. Decoction is an important measure to decrease toxicity and reserve efficacy. In the course of decoction, the ester alkaloids have the complex changes such as dissolving-out, hydrolyzing, converting and so on. The diester-type alkaloids convert into mono-ester type alkaloids and alkylol amine alkaloids. Meanwhile, the composition and contents of toxicity and effective components were changed. Therefore, the decocting time is closely linked with the clinical effect and poison side-effect.Aiming at these problems, this thesis includes the following four sections.(1) Determination of total ester-alkaloids in Radix Aconiti Lateralis Praeparata. The diester-type alkaloids and mono-ester type alkaloids both contain benzoyl ester, so the benzoic acid generated after alkaline hydrolysis. The benzoic acid were determined and the contents multiplied by the coefficient of 5.287 7 (the ratio of molecular weight between aconitine and benzoic acid) were equivalent to the contents of total ester alkaloids (calculated based on aconitine). This method is simple, reliable and provides a determination method of total ester -alkaloids in aconites medicinal materials.(2) Study on HPLC Fingerprinting and determination of six kinds of ester-type alkaloids of Radix Aconiti Lateralis Praeparata. We established the HPLC fingerprint spectrum of Baifupian, Heifupian and determined six kinds of ester-type alkaloids simultaneously. This method with multi-component and multi-index provided scientific basis to standardize preparing technology and use Radix Aconiti Lateralis Praeparata reasonably and safely.(3) Determination of total ash, acid-insoluble ash and total inorganic salt in Radix Aconiti Lateralis Praeparata. EDTA titration was used to determine the contents of Magnesium, Calcium and others metal ions and the total inorganic salt were measured with magnesium chloride. Meanwhile, the total ash and acid-insoluble ash were determined for indirect control the residues of DanBa and other inorganic salt. This method is simple, reliable and can be used for the quality control for Radix aconiti lateralis praeparata slice.(4) Study on dynamic variation patterns of ester alkaloids in Radix Aconite Lateralis at different decocting time. Unprocessed Radix Aconiti Lateralis and Baifupian were the objects of study. We studied the dynamic variation patterns of ester alkaloids in the decocting process combined with hvdrolvsis of reference substances.