Effects Of FZD2and FZD3 Genes On The Proliferation And Invasiveness Of Salivary Adenoid Cystic Carcinoma Cell

Salivary Adenoid Cystic Carcinoma (SACC) is one of the most common malignant tumors in parotid gland. It tends to be frequently recurrent after surgery due to the special invasiveness and metastasis ability. Novel moleculars related to the invasiveness and metastasis of SACC can help us to seek for the methods to diagnose and treat the tumor and predict its development tendency.Objective Based on the previous results which showed the de-regulation of FZD2 and FZD3 in SACC cells and tissues, the aim of present study is to explore the effct of FZD2 and FZD3 on the proliferation and invasiveness of Salivary Adenoid Cystic Carcinoma. Methods Part I Small interference RNAs (siRNA) were used to knock down the expression of FZD2 and FZD3 in SACC-M cells.The expressions of FZD2 and FZD3 genes were analyzed by Western blot,cell growth was measured by CCK-8 kit,and cell invasiveness was examined by transwell chambers.Part II The FZD2 and FZD3 recombinant adenoviral expression vector (Ad-FZD2 and Ad-FZD3) was constructed using AdEasy XL Adenoviral Vector System. The expressions of FZD2 and FZD3 genes were analyzed by Western blot,cell growth was measured by CCK-8 kit,and cell invasiveness was examined by transwell chambers.Result Part I After transfection of siRNAs for 48 hours, the expression of FZD2 and FZD3 was effectively down-regulated as shown by Western blot. The proliferation and invasion ability of SACC-M cells were enhanced when the expressions of FZD2 and FZD3 genes were inhibited in SACC-M cells.Part II After 48 hours when SACC-M cell was infected with MOI=100 recombinant adenoviruses, Western blot showed that the protein expressions of Ad- FZD2 and Ad-FZD3 groups were significantly increased compared to the negative control group. The proliferation and invasion ability of SACC-M cells were inhibited by over-expressions of FZD2 and FZD3 genes.Conclusions These results suggest that FZD2 and FZD3 genes may play key roles in human SACC. The expressions of FZD2 and FZD3 genes may inhibit the proliferation and invasiveness of SACC. Further study may provide more convencing evidences of the roles of FZD2 and FZD3 in the genesis and progress of SACC.