Correlation Of The Expression Of E-Cadherin And CyclinE In Cervical Squamous Cell Cancer

ObjectiveThe study aimed to investigate the expression of E-cadherin and Cyclin-E in normal cervical tissue, the tissues of cervical intraepithelial neoplasia and cervical squamous cell carcinoma, and to elucidate the function and significance of E-cadherin and Cyclin-E in the occurrence and development of cervical squamous cell carcinoma.MethodsFrom June 2006 to June 2009 in Qing Yuan city people's hospital,80 cases of specimens by surgery or cervical biopsy and cervical lesions paraffin specimens in ourpathologicaldepartment were recruited. Including:(1) 60 cases of cervical intraepithelial neoplasia (CINⅠ20 cases, CINⅡ20 cases, CINⅢ20 cases); (3) 20 cases of cervical squamous cell carcinoma in 20 patients (stage I 10 cases, stage II 7 cases, stageⅢ3 cases; 7 cases with moderate different Tilted squamous cell carcinoma,13 patients with low differentiated squamous cell carcinoma, no high differentiated squamous cell carcinoma). The ages of the patients were from 24 years old to 73 years, the average age was 41.55±11.46 years old.Another 20 control cases were from the whole hysterectomy due to uterine momma or and uterine adenoma. The ages were from 27 to 62 years old, the average age was 34.93±6.02 years old.No special or vaginal treatments were given for the patients before operation or cervical biopsies, the clinical and pathological data were available for all the cases. After fixation by 10% formaldehyde solution, living cervical specimen were made to paraffin embedding specimens.From each specimen,4 paraffin slices with 4μm thick were obtained, one for pathological diagnosis with HE stain, the other two slides were detected with immunohistochemical method (S-P method) for expression of E-cadherin and cyclin-E, the last one for spare.Results1, E-cadherin (E-cadherin) expression:in the normal control group, E-cadherin expression in the cell membrane, showing a continuous expression of brown, less stained cytoplasm and nucleus, the positive cells was 100%; in cervical intraepithelial neoplasia group of E-cadherin membrane expression was decreased in the cytoplasm see some expression of E-cadherin, the positive cells was 75%; in squamous cell membrane and cytoplasm little expression of E-cadherin, the positive cells was 35%. E-cadherin in the three groups of positive cells increased from normal cervical tissue→CIN→cervical squamous cell carcinoma showed a declining trend, the three groups was statistically significant P＜0.05.2, CyclinE expression:CyclinE mainly expressed in the nucleus, little expression in the cytoplasm. In the normal control group, there was no expression of CyclinE, the positive cells was 0%; in cervical CIN group, Cyclin-E expression in the nucleus increased, the positive cells was 32%; in squamous cell carcinoma group to further increase the expression of CyclinE, the positive cells was 60%. CyclinE in the three groups in the expression of positive cells from normal cervical tissue→CIN→cervical squamous cell carcinoma showed increasing tendency among the three groups was statistically significant P＜0.05.3, in cervical CINⅠ, CINⅡand CINⅢgroups in the expression of E-cadherin positive cells were 100%,70%,55%, three between the two groups P＜0.05, statistically significant. The CyclinE expression positive rates were 10%,35%and 50%. Comparison among the three groups P＜0.05, statistically significant. 4, E-cadherin expression in different stages of cervical cancer in the percentage of positive cells, respectively, I of 30%,Ⅱof 43%,Ⅲof 33%between the three groups was not significant P＞0.05. The CyclinE ratio of positive cells were 50%,57%and 100%, three between the two groups was not significant P> 0.05.Conclusions1, E-cadherin in cervical tissues, cervical intraepithelial neoplasia and cervical squamous cell carcinoma showed gradual decreasing trend, indicating that the occurrence and development of cervical cancer may be associated with reduced expression of E-cadherin-related.2, CyclinE in squamous cell carcinoma was higher than normal cervical tissue were also higher than cervical CIN groups, indicating that up regulation of CyclinE of the development may be associated with cervical squamous cell carcinoma.3, E-cadherin expression decreased with the increased expression of CyclinE, Both in the cervical squamous cell carcinoma had no correlation.4, E-cadherin in 100% of normal cervical tissue expression, and CyclinE no expression in normal cervical tissue, cervical tissue if the detected E-cadherin, or abnormal expression of CyclinE, then these indicators may be used as a molecular abnormal cervical cells biological indicators.