The Expression Of Centromere Protein A In Non-small Cell Lung Cancer And Its Clinical Significance

Objective:To study the different expression of centromere protein A (CENP-A) in human non-small cell lung cancer (NSCLC) and lung tissues of benign lesions.To see the relationship between the clinical characteristics of patients and the expression of CENP-A.To investigate the relationship between the survival time and the expression of CENP-A. In order to find a new biomarker for judging the prognosis of NSCLC patients or a new target point for the therapy of NSCLC.Methods:This study retrospectively analyzed the clinical and pathological data of 66 NSCLC patients after surgical treatment.The expressions of CENP-A in 66 cases of human non-small cell lung cancer and 20 cases of benign lung tissue samples archived in paraffin blocks are controlled by the En Vision Immunohistochemical method.66 cases of NSCLC by collecting basic clinical information in hospital, according to the clinical laboratory examinations and surgery; by way of telephone or mail follow-up, the survival time of postoperative patients is obtained. SPSS18.0 statistical software is used to analyze CENP-A expression and clinical data, pathologicaldata of NSCLC patients by using the X2 test, the cumulative survival rate is calculated by Kaplan-Meier method of statistical and the Cox proportional hazards model analysis of the prognosis of NSCLC patients factors.Results:CENP-A in NSCLC samples located in the nucleus, the expression of the positive rate was 65.15%, benign lung diseases were negative in the expression of CENP-A,and the difference between them was significant (P<0.05). The expression of CENP-A in total positive rate in well differentiated groups was 38.89%, in moderately+poorly differentiated groups was 75.00%, difference between the groups was significant (P< 0.05). In the higher degree of differentiation, CENP-A expression in the total positive rate is low. The total positive rate of CENP-A expression in smoking patients (75.68%) and non-smoking patients (51.72%) were significant difference (P<0.05).CENP-A positive rate of expression was no significant difference (P> 0.05) in the patient's age, gender, tumor size, lymph node metastasis, and TNM staging.COX proportional hazards regression model showed that survival time of patients was significantly associated with CENP-A expression and lymph nodes metastasis (P<0.05). Kaplan-Meier survival curves suggested that more than 3 years survival time of NSCLC patients was significantly different between positive and negative expression of CENP-A (P<0.05).Conclusion:1.The expression of CENP-A in human NSCLC is in high level, but negative expression in benign pulmonary lesions 2.The positive expression of CENP-A in the high degree of differentiation of NSCLC patients was significantly lower than patients with low degree of differentiation. Positive expression of CENP-A smoking patients was higher than non-smokers.3.CENP-A expression in patients with NSCLC survival rate of less than 3 years of negative expression of CENP-A patients.4.lymph nodes metastasis and positive expression of CENP-A were considered an independent predictor for the prognosis of NSCLC.