| Background:This study examined single nucleotide polymorphisms in IL-1βand PSCA and their associations with risk of developing gastric cancer in relation to Helicobacter pylori infection in Chinese Han population.Methods:We conducted a hospital-based case-control study including 221 incident gastric cancer patients and 190 gastritis controls. Serum levels of anti-H.pylori IgG were measured by enzyme-linked immunosorbent assay to indicate H.pylori infection The single nucleotide polymorphisms (SNPs) in the IL-1β-31, IL-1β-511 and PSCArs2294008,PSCA rs 2976392 were analysised by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results:1. The H.pylori infection rate is 70.6% in gastric carcinoma patients compared to 76.3% in gastritis patients. There is not significant difference between these two groups in infection rate (P>0.05). There are no statistical difference in the rate of Hpylori infection between IL-1β-31,IL-1β-511 and PSCA rs2294008,PSCA rs2976392 TT(AA),CT(AG),CC(GG) genotypes CP>0.05)2. IL-1β-31CC genotype showed an increased risk for gastric cancer (OR=2.24,95%CI: 1.02-4.93, P=0.045) while an elevated risk of gastric cancer was observed in subjects with H.pylori infection (OR=2.38,95%CI:1.15-4.91,P=0.020)3. IL-1β-511 polymorphisms was are not associated the risk of gastric cancer in this study.4. PSCA rs2294008 CT and TT genotype showed an increased risk of gastric cancer in gastric cancer patients and gastritis controls. (OR=1.54,95%CI:1.01-2.35,P=0.04; OR=2.79,95%CI: 1.27-6.13, P=0.01). While no elevated risk of gastric cancer was observed in subjects with H.pylori infection. Furthermore, PSCA rs2294008 TT genotype showed an increased risk for gastric cancer in gastric cancer patients and umbilical cord blood controls. (OR=2.43,95%CI:1.05-5.63,P=0.04).5. PSCA rs2976392 AA genotype gene showed an increased risk for gastric cancer in gastric cancer patients and the umbilical cord blood controls. (OR=2.96,95%CI:1.05-8.32, P=0.04) while no association was found in gastritis controls.Conclusions:①No significant difference was found between gastric carcinoma patients and gastritis patients in H.pylori infection rate in this study.②The SNPs (IL-1β-31,IL-1β-511 and PSCA rs2294008,PSCA rs2976392) may not produce a marked effect in H.pylori infection.③IL-1β-511 polymorphism could not infect the risk of gastric cancer. Genetic polymorphisms in IL-10-31 may interaction with H.pylori infection and may play important roles in developing gastric cancer. Genetic polymorphisms in PSCArs2294008,PSCArs2976392 showed a relationship with the risk of gastric cancer in this study, but no intraction was found between the polymorphism and H. pylori infection.
|
PDF Full Text Request