Relationship Between Hhcy And No-reflow After Reperfusion Of Rat AMI

Objective:Hhcy is the serum total homocysteine concentration in such pathological conditions.Large number of clinical and epidemiological evidence suggested that Hhcy is an independent risk factors for atherosclerosis disease.Acute myocardial infarction (AMI) is a common one of critically illness, thrombolysis, percutaneous coronary intervention and coronary artery bypass grafting are widely used in clinical.therapy of AMI into the reperfusion period, but Some patients with no-reflow phenomenon (NRP) after revascularization, which seriously affected the prognosis of patients.Recently, there was little the report that Hhcy affected reperfusion of AMI no-reflow phenomenon. this study began with the preparation of Hhcy and Hhcy ischemia-reperfusion model, change from cardiac markers, oxidative stress, vascular factors and ECG, the mechanism for the Hhcy involved AMI No-reflow after reperfusion, for the Research and Prevention of no-reflow after ischemia-reperfusion following AMI to provide new data.Methods:①By 2% L-cysteine intraperitoneal injection established rat Hhcy model and coronary artery ligation model of acute myocardial ischemia;②The rats were divided into control group, hyperhomocysteinemia group, ischemia reperfusion group and Hhcy +ischemia reperfusion group; Enzyme-linked immunosorbent assay serum Hcy concentration of groups.④Measured by Serum CK and LDH;⑤Measured by colorimetry and peroxidation of serum and myocardial concentrations of malondialdehyde and superoxide dismutase, glutathione peroxidase activity in serum;⑥Double-antibody sandwich ELISA assay and immune-related inflammatory response of serum C-reactive protein;⑦Double-antibody sandwich ELISA assay of serum endothelin-1, angiotensinⅠ, angiotensinⅡand MPO.Result:①rat Hhcy model and ischemia reperfusion model were successfully established.②ECG were significantly changed pre- and post-myocardial ischemia reperfusion, and Hhcy+ischemia-reperfusion group appeared arrhythmia of 4 cases.③Hhcy can lead to further myocardial injury after reperfusion, it showed that the serum CK, LDH were significantly increased.④Hhcy made the rats body in the oxidation state, the performance of the serum SOD, GSH-Px activity decreased and MDA content increased.⑤Hhcy increased the production and release of vasoactive factor ET-1, AngⅠ, AngⅡ, CRP and MPO.Conclusion:The experimental from the many aspects of the Hhcy model and the Hhcy model of myocardial ischemia preparation to change from ECG before and after AMI and postreperfusion to electrocardiographic changes after reperfusion immunohistochemical analysis the NRP mechanism from Hhcy lead AMI after reperfusion. Our study shows:It can estable the stable animal model of hyperhomocysteinemia by Intraperitoneal injection of 2% L-cysteine 5ml/kg after 10 weeks; Left ventricular descending branch of coronary artery ligation for 60min and release the ligature after reperfusion 60min can reproduced stable animal model of myocardial ischemia reperfusion.Postreper-fusion, myocardial injury aggravated, electrocardiogram changes significantly, CK and LDH elevated; oxidative stress, production and release of vasoactive factors are the mechanism of Hhcy cause AMI postreperfusion further damage. Our study for further research and relationship between Hhcy and the NRP and lay the foundation for the Prevention occurrence of no-reflow after reperfusion following AMI.