| Objective: To observe the effects of hyperhomocysteinemia on no-reflowafter acute myocardial infarction reperfusion in rats, evaluate the effects oftrimetazidine on no-reflow after acute myocardial infarction reperfusion inhyperhomocysteinemia rats, and investigate the mechanism of it.Methods: Forty-six SD rats were randomly divided into the blank group(10), the contral group (12), the Hhcy model group (12) and the trimetazidineintervention group (12),♀♂were in equal portions. During the feeding period,saline5ml/Kg was given by intraperitoneal injection and intragastric adminis-tration in the blank group and the contral group every day.2%L-cysteine5ml/Kg was given by intraperitoneal injection and saline5ml/Kg was given byintragastric administration in the Hhcy model group every day.2%L-cysteine5ml/Kg was given by intraperitoneal injection and trimetazidine6.3mg/Kgwas given by intragastric administration in the trimetazidine intervention groupevery day.After12weeks continuous dosing, anesthetized rats and connectedthe small animal ventilator. Exposed heart and ligated the left ventriculardescending artery for90minutes, and then reopened the artery, in the blankgroup there were only thread without ligation. After90minutes of reperfusion,two rats were randomly selected and stained the myocardium by Evan’s blue,then calculate the range of no-reflow. The rest rats were sacrificed to take theplasma of arterial blood, and measured the level of Hcy, TN-I, SOD, MDA,CAT, sICAM-1and P-S. While observing the ECG changes in the wholeischemia and reperfusion process. Results:①The levels of plasma Hcy. The levels of plasma Hcy in theHhcy model group were significantly increased compared with that in thecontral group (P<0.001), whereas those had no significant difference in thetrimetazidine intervention group (P>0.05).②The changes of ECG.At90minutes after ligation of LCX the R wave reduced and the ST segment elevatedsignificantly compared with ligation before (P<0.05). At90minutes afterreperfusion the ST-segment fell down significantly in the trimetazidineintervention group compared with90minutes after the ligation of LCX (P<0.05),but there was no significant changes in the contral group and the Hhcy modelgroup (P>0.05).③Range of no-reflow.The no-reflow range in the contral groupis16.15%、19.37%. The no-reflow range in the Hhcy model group is34.47%,27.37%. The no-reflow range in the trimetazidine intervention group is12.71%、13.37%.④The levels of plasma TN-I. The levels of plasma TN-I in the Hhcymodel group were significantly increased compared with that in the contralgroup (P<0.001), whereas those were significantly decreased in thetrimetazidine intervention group (P<0.001).⑤The activity of plasma SOD,CAT. The activity of plasma SOD, CAT in the Hhcy model group weresignificantly decreased compared with that in the contral group (P<0.001),whereas those were significantly increased in the trimetazidine interventiongroup (P<0.001). The level of plasma MDA.The level of plasma MDA in theHhcy model group were significantly increased compared with that in thecontral group (P<0.001), whereas those were significantly decreased in thetrimetazidine intervention group (P<0.001).⑥The expression of plasmas-ICAM and P-S. The expression of plasma s-ICAM and P-S in the model groupwere significantly increased compared with that in the contral group (P<0.001),whereas those were significantly decreased in the trimetazidine interventiongroup (P<0.001). Conclusion:1A stable SD rat model of hyperlipidemia can be establishedby continuous intraperitoneal injection of2%L-cysteine5ml/Kg·d for12weeks;2A stable acute myocardial infarction reperfusion model can beestablished by SD rat left ventricular coronary descending artery90min ligationand releasable ligature for90min.3No-reflow phenomenon is confiremed bymyocardial Evans blue staining after myocardial infarction reperfusion in acutemyocardial infarction reperfusion model.4Hhcy maybe a factor of increasingthe no-reflow after myocardial infarction reperfusion, it can increase the rangeof no-reflow after myocardial infarction reperfusion.5Trimetazidine caneffectively reduce the range of no-reflow after acute myocardial infarctionreperfusion in hyperlipidemia rats, the mechanism maybe related to reducesoxidative stress and inflammation. |
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