| Background:Paraquat (paraquat, PQ) also known as Gramoxone, paraquat, Balally, chemical name 1,1 '-dimethyl 4,4'-dichloro-b pyridine, bipyridine are heterocyclic compounds, Herbicide is exposed, is one of the most widely used herbicide, the case base decomposition, rapid loss of activity after contact with soil, paraquat is highly toxic to humans and animals with acute poisoning mortality rate as high as 50% -80%[1], causes high mortality and pathogenesis is unclear and no effective antidote clinical drugs. Lung, liver, kidney, heart, etc. are the target organ of paraquat poisoning injury. Can be expressed as in the early acute kidney injury can be manifested as severe acute renal failure and even death. Kidney is the body's vital organs of life, has many physiological functions, mainly to excretory function and regulate water and electrolyte, acid-base balance, and can regulate blood pressure, kidneys secrete renin, erythropoietin and other hormones. When paraquat poisoning, the kidneys as excretory organs, first of all damage, leading to all kinds of dysfunction. Since most of the toxic substances in the body kidney, and so paraquat poisoning, paraquat poisoning, a large number of toxic and inflammatory substances excreted. When an abnormal kidney function, although the urine may appear to be normal, but the excretion of toxic dysfunction, leading to paraquat and inflammatory substances in the body can not effectively discharge to the liver, lung lesions are promoted, thereby affecting Herbs dry poisoning prognosis. It can be seen, PQ poisoning treatment of renal injury in a very important position, full of kidney function is to ensure that the basis for effective treatment of other organs.Objective:clinical practice, the use of UTI have renal protective effects of the treatment, but less specific effect of the treatment; and on the clinical treatment of UTI the dose is no clear basis for choice, this study of paraquat (paraquat, PQ) poisoning pathological features of renal injury, and through the effect of different doses of UTI treatment of renal injury PQ poisoning effect of UTI in the optimum dose, can initially achieve the following objectives:(1) clearly paraquat poisoning characteristics of renal disease at different times; (2) clearly UTI treatment of PQ poisoning, the role of the kidney and its dose-effect relationship.Methods:Wistar rats were chosen and 70 laboratory, by the Experimental Animal Center of Jilin University, irrespective of gender, body weight (250+20) g, the rats were randomly divided into 7 groups, including through the preparation of six groups of rats PQ poisoning by mouth model, respectively,2 times a day to give A:normal saline 1ml twice daily intraperitoneal injection; B group:treated group:daily intraperitoneal injection of normal saline 1ml; C group:treated group:Ulinastatin D 30,000 iu/kg twice daily intraperitoneal injection; D groups:treated group:UTI 60,000 iu/kg intraperitoneal injection twice daily; E group:treated group:UTI 90,000 iu/kg intraperitoneal injection twice daily; F groups:treated group:UTI 120000 iu/kg twice daily intraperitoneal injection; G groups:treated group:UTI 180000 iu/kg per day Two intraperitoneal injection, blood was collected in the first 1,3,7,14 days serum creatinine values, on the first day to take the kidney 1,3,7,14,21,28, HE staining.Results:1, severe kidney injury, renal tubular lesions in the main to the main acute and chronic phase gradually restored. PQ poisoning through the observation of renal HE staining, the lesions found in group B the most serious bleeding manifested as glomerular and tubular protein cloudy swelling visible lumen tube, tubular epithelial cells occasionally hyaline degeneration, interstitial inflammation infiltration, some tubular necrosis, structural collapse, showing interstitial hemorrhage.2, C, D, E, F, G group of kidney disease in the exposed group was significantly lighter, C, D, E, F, G set the value of the group increased creatinine level of exposure was significantly lower than the control group.3, F, G group of kidney disease is lighter than C, D, E group, the acute phase significantly, chronic little difference. Conclusion:1, paraquat poisoning, serious kidney damage, and its tubular lesions in the main, the specific pathology showed significant swelling of renal tubular epithelial cells, glass-like stools, see a large number of renal interstitial inflammatory infiltration and hemorrhage also visible in the tubular cavity and bleeding protein casts, and glomerular injury minor, can see a small amount of bleeding renal capsule. Paraquat poisoning in acute renal injury is most obvious in the chronic phase gradually reduced.2 UTI on renal injury in paraquat poisoning have significant therapeutic effect, can effectively reduce the degeneration of renal tubular epithelial cells, and can reduce the infiltration of renal interstitial inflammation and bleeding, and can effectively inhibit the increase of creatinine. 3, the larger dose of UTI on the therapeutic effect of paraquat poisoning in acute renal relatively small dose of good, there are significant differences, more than 7 days after the differences between the groups was reduced.
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