Tnf-α Expression Of Ischemia-reperfusion Rabbit Renal And Renal Hemodynamics Correlation

Objective To observe tumor necrosis factor (TNF-α) expression in rabbit renal ischemia-reperfusion (I/R) had correlation with the renal artery hemodynamics changes to use color doppler flow imaging (CDFI) to evaluate and provide a feasible method to non-invasive, timely evaluate the occurrence of renal ischemia reperfusion injury(IRI). Methods 48 rabbits were randomly divided into sham operation group, I / R group. Sham group and the I/R group were divided into 2 h, 8 h, 24h 3 subgroups according different reperfusion time point and each subgroup 8. Each group of rabbits were anesthesied with 2% sodium pentobarbital through the ear vein injection(dosage 1.5ml/kg ). After the success of anesthesia, the hair of left kidney area was removed and try skin to exposed. The right kidney were all removed both sham operation and ischemia-reperfusion groups. In the I/R group,the renal arteriovenous were occlusioned with the artery clamp,and resulted in renal ischemia, after renal ischemia for 1h, and restoration of perfusion were recovered. Application of CDFI in each group left renal master artery (MRA),renal segment artery (SRA), renal interlobar artery(IRA) blood flow dynamics were monitore .Determination of renal arterial blood flow in each group had been finished, the left kidney were removed in the low temperature state. left kidney tissue were used to measure the renal tissue TNF-αactivity . by immunohistochemistry (SABC) measured the average optical density (OD) of TNF-αof rabbit kidney tissue . the average optical density (OD) of TNF-αof the I / R group at each time point correlated with renal artery blood flow dynamics was analyzed. The hemodynamic parameters were tested through homogeneity of variance, and undertaking ANOVA analysis (P<0.05 was regared as statistically significance); three levels renal artery Vmax, Vd, PI, RI and the relationship between TNF-αOD were analyzed by linear correlation and regression to calculate the correlation coefficient r value (P<0.05 as statistically significant). Results (1) Hemodynamics changes are as follow:①Sham operation 2h subgroup: master renal artery Vmax, Vd, PI, RI were 43.20±5.65 (cm / s), 23.85±4.37 (cm / s), 0.58±0.08,0.42±0.06; segmental artery Vmax, Vd, PI, RI were 28.98±7.56 (cm / s), 16.91±4.32 (cm / s),0.50±0.05, 0.38±0.08; interlobar artery Vmax, Vd, PI, RI were 19.43±5.89 (cm / s), 12.86±3.14 (cm / s), 0.47±0.13 , 0.35±0.07;②Sham operation 8h subgroup: master renal artery Vmax, Vd, PI, RI were 41.74±7.20 (cm / s), 22.97±5.14 (cm / s), 0.57±0.10,0.41±0.10; segmental artery Vmax, Vd, PI, RI were 27.12±6.81 (cm / s), 16.22±5.73 (cm / s), 0.49±0.07, 0.37±0.10; interlobar artery Vmax, Vd, PI, RI were 18.34±5.17 (cm / s), 12.62±4.62 (cm / s), 0.46±0.11,0.35±0.11;③Sham operation 24h subgroup: master renal artery Vmax, Vd, PI, RI were 42.98±5.44 (cm / s), 23.58±7.08 (cm / s), 0.57±0.11,0.42±0.08; segmental artery Vmax, Vd, PI, RI were 28.65±7.43 (cm / s), 17.56±6.13 (cm / s), 0.49± 0.10, 0.38±0.06; interlobar artery Vmax, Vd, PI, RI were 19.71±3.57 (cm / s), 13.32±4.54 (cm / s), 0.47±0.09,0.35±0.08 .④Ischemia-reperfusion 2h subgroup: master renal artery Vmax, Vd, PI, RI were 46.23±4.53 (cm / s), 24.36±6.19 (cm / s), 0.67±0.18,0.47±0.14; segmental artery Vmax, Vd, PI, RI were 30.40±3.15 (cm / s), 18.52±2.86 (cm / s), 0.61±0.10,0.44±0.09; interlobar artery Vmax, Vd, PI, RI were 20.65±3.83 (cm / s), 11.92±3.27 (cm / s), 0.59±0.08,0.40±0.07;⑤Ischemia-reperfusion 8h subgroup: master renal artery Vmax, Vd, PI, RI were 48.17±7.05 (cm / s), 24.88±6.79 (cm / s), 0.75±0.19,0.49±0.08; segmental artery Vmax, Vd, PI, RI were 34.25±6.18 (cm / s), 18.36±4.16 (cm / s), 0.72±0.16,0.48±0.07; interlobar artery Vmax, Vd, PI, RI were 23.26±5.25 (cm / s), 11.69±3.84 (cm / s), 0.70±0.18 , 0.45±0.10.⑥Ischemia-reperfusion 24h subgroup, master renal artery Vmax, Vd, PI, RI were 55.11±4.34 (cm / s), 20.60±7.87 (cm / s), 1.09±0.54,0.63±0.13; segmental artery Vmax, Vd, PI, RI were 37.71±3.53 (cm / s), 17.67±2.99 (cm / s), 0.97±0.14,0.57±0.11; interlobar artery Vmax, Vd, PI, RI were 28.66±5.81 (cm / s), 9.73±2.97 (cm / s), 0.89±0.32, 0.54±0.09; Compared with sham-operated group, and I/R 2 h subgroup had no significant changes in peak systolic velocity subgroups (Vmax), end diastolic velocity (Vd), pulsatility index (PI) and resistance index. I/R 8 h subgroup, RI of rabbits SRA and IRA increased (both P<0.05), and in I/R 24 h subgroup, Vmax, PI, RI of rabbits MRA, SRA and IRA increased (all P<0.05). Compared with OD of TNF-α(0.1302±0.027) of sham-operated group 2h subgroup, OD of TNF- α(0.1607±0.033) of I/R 2h subgroup was no significant difference; Compared with OD of TNF-α(0.1321±0.030) of sham-operated group 8h subgroup, OD of TNF-α(0.1405±0.028)of sham-operated group 24h subgroup , I/R8h subgroup TNF-αOD (0.1879±0.035), I/R24h subgroup TNF-αOD (0.2658±0.040) were statistically significant (P all <0.05); and increased within 24 h (P <0.05). The master renal artery Vmax, PI, RI and renal tissue TNF-αOD was positively correlated (r=0.714,0.764,0.782,P<0.05), and Vd was no significant correlation (P>0.05). The renal segmental artery of the Vmax, PI, RI and renal tissue TNF-αOD was positively correlated (r=0.779,0.811,0.821,, P<0.05), and Vd was no significant correlation (P>0.05). The renal interlobar artery Vmax, PI, RI and renal tissue TNF-αOD was positively correlated (r=0.833,0.788,0.828,P<0.05), and Vd was no significant correlation (P>0.05). TNF-αOD for I/R group would be independent variable as x, to renal master artery of Vmax as the dependent variable y, for linear regression analysis, linear regression equation: Y=72.86X+33.23. TNF-αOD for I/R group would be independent variable as x, to renal segment artery of Vmax as the dependent variable y, for linear regression analysis, linear regression equation: y=71.17x+19.44. TNF-αOD for I/R group would be independent variable as x, to renal interlobar artery of Vmax as the dependent variable y, for linear regression analysis, linear regression equation: y=81.21x+7.821 . TNF-αOD?for?I/R?group?would?be?independent?variable?as?x,to?renal?master?artery?of?PI?as?the?dependent?variable?y,?for?linear?regression analysis,?linear?regression?equation:?Y=5.007X‐0.196. TNF-αOD for I/R group would be independent variable as x, to renal segment artery of PI as the dependent variable y, for linear regression analysis, linear regression equation: y=4.148x-0.084. TNF-αOD for I/R group would be independent variable as x, to renal interlobar artery of PI as the dependent variable y, for linear regression analysis, linear regression equation: y=3.272x+0.050 . TNF-αOD?for?I/R?group?would? be? independent? variable? as x,? to? renal segment? artery? of? RI? as? the?dependent?variable?y,?for?linear?regression?analysis,?linear?regression?equation: y=1.487x+0.193 . TNF-αOD?for?I/R?group?would?be?independent?variable as x, to?renal?interlobar artery?of RI as the dependent variable y,?for?linear?regression?analysis,?linear?regression?equation:?y=1.452x+0.166 .Conclusion Compared with the sham operation group, after ischemia and reperfusion 8h,rabbit renal artery resistance index began to increase and the first occurred in the peripheral blood vessels; after ischemia and reperfusion 24h ,all levels of rabbit renal artery occurred Vmax, PIand RIwere increased. Compared with the sham operation group, after ischemia and reperfusion 8h, TNF-αbegan to increase and there are more severe trend in the 24h. I / R subgroups changes in rabbit renal artery Vmax, PI,RI and TNF-αof rabbit kidney tissue has a positive correlation. Using CDFI to detect the renal artery blood flow dynamics of renal ischemia reperfusion is a feasible method to non-invasive, timely evaluate the occurrence of renal ischemia reperfusion injury.